RT info:eu-repo/semantics/article
T1 Assessment of lipid uptake and fatty acid metabolism of European eel larvae (Anguilla anguilla) determined by 14C in vivo incubation
A1 Pérez Pérez, José Antonio
A1 Lund, Ivar
A1 Reis, Diana B.
A1 Tomkiewicz, Jonna
A1 Benini, Elisa
A1 Kottmann, Johanna S.
A1 Politis, Sebastian N.
A1 Rodríguez, Covadonga
A2 Biología Animal y Edafología y Geología
A2 Grupo de investigación NUTRAHLIPIDS (Fisiología de los Lípidos y sus Derivados en la Alimentación Animal y Humana)
K1 European eel larvae
K1 PUFA metabolism
K1 Phospholipids
K1 Radiolabelled substrates
K1 Arachidonic acid
AB Knowledge on dietary nutrient requirements of first-feeding European eel larvae (Anguilla anguilla) is verylimited. This study provides first ever information on in vivo lipid uptake and fatty acid (FA) metabolism ofEuropean pre-leptocephalus eel larvae and advances directions for dietary lipid and FA inclusions. The in vivocapability of eel larvae to incorporate and metabolize unsaturated fatty acids was tested on larvae at differentontogenetic stages (4, 8 and 12 days post hatch, DPH). Larvae were incubated in 10 mL flat-bottom tissue cultureplates, with [1-14C]-labelled FA (18:2n-6, ALA; 18:3n-3, LA; 20:4n-6, ARA and 20:5n-3, EPA) directly added toseawater. The capability of the larvae for de-acylation and re-acylation of [1-14C]arachidonic acid (ARA), initially bound to phosphatidylcholine (PC) and phosphatidylethanolamine (PE), was also investigated. In allcases, control incubations without any radiolabelled substrate were performed for further lipid analysis. Theresults revealed that direct incubation with 14C-labelled FA is a feasible method to investigate in vivo FA andphospholipids metabolism of pre-leptocephalus stages of the European eel. No enzymatic elongation/desaturation activity towards [1-14C]C18 or [1-14C]C20 FA was detected. Consequently, ARA, eicosapentaenoic acid(EPA) and docosahexaenoic acid (DHA) must be considered essential FA and thus provided firstly through female broodstock and later through diet at least during the first-feeding stage. Pre-leptocephalus larvae display ahigh capacity to remodel dietary phospholipids with a preferential esterification of all FA substrates into PC. Theunexpectedly high esterification rate of [1-14C] ARA into PC and PE is supported by the individual FA profiles ofthe larval phospholipids. The high levels of ARA present in the European eel larvae denotes its physiologicalrelevance for this species. It is therefore essential to consider this FA as particularly important when designingsuitable broodstock – or first-feeding diets for this species.
YR 2021
FD 2021
LK http://riull.ull.es/xmlui/handle/915/35413
UL http://riull.ull.es/xmlui/handle/915/35413
LA en
NO https://doi.org/10.1016/j.aquaculture.2020.735858
DS Repositorio institucional de la Universidad de La Laguna
RD 16-ago-2024