RT info:eu-repo/semantics/article
T1 Fatty acid profiles and omega-3 long-chain polyunsaturated fatty acids (LC-PUFA) biosynthesis capacity of three dual purpose chicken breeds
A1 Pérez Pérez, José Antonio
A1 P´erez, José A.
A1 Castro, Almudena
A1 Rolo, Carla
A1 Torres, Alexandr
A1 Dorta-Guerra, Roberto
A1 Acosta, Nieves G.
A1 Rodríguez, Covadonga
K1 Elongation
K1 Desaturation
K1 Lipid metabolism
K1 Dual purpose chicken
K1 Omega-3
K1 Long chain polyunsaturated fatty acids
K1 EPA
K1 DHA
AB Westernized societies ingest an unhealthy high dietary omega-6/omega-3 fatty acid ratio of 20:1 or even higher.Seafood is the primary source of omega-3 long chain polyunsaturated fatty acids (LC-PUFA) for humans, mainlyeicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), which are involved in a plethora of physiologicaland health-related processes. However, the production of marine organisms with aquafeed formulations based onmarine ingredients leads to serious environmental impacts on global pelagic fish resources, resulting in an unsustainable activity. The present study aims to gain further insight into the metabolism of fatty acids in chickenas a potential supply for omega-3 LC-PUFA. To this purpose, lipid classes and fatty acid profiles of thighs andhepatocytes, and the modification of [1-14C]18:3n-3 by hepatocytes isolated from three dual-purpose chickenbreeds adapted to free-range culture systems were determined. Arachidonic acid highly accumulated in thighsmeat (7.16–8.79 %) despite being barely supplied in the diet, with DHA (1.22–1.71 %) and n-3 docosapentaenoicacid (DPA, 22:5n-3; 1.02–1.14 %) being also relevant. Our experimental design with radiolabeled fatty acids wasvalidated for the first time in terrestrial vertebrates. Chicken hepatocytes incubated with [1-14C]18:3n-3 produced a wide variety of C18-C24 intermediates demonstrating that the set of fatty acyl desaturases and elongasesenzymes necessary to metabolize dietary C18 precursors are active for the production of LC-PUFA, includingEPA, n-3 DPA and DHA.
SN 0889-1575
YR 2021
FD 2021
LK http://riull.ull.es/xmlui/handle/915/35414
UL http://riull.ull.es/xmlui/handle/915/35414
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 09-may-2024