RT info:eu-repo/semantics/article
T1 Influence of salinity and linoleic or ¿-linolenic acid based diets on ontogenetic development and metabolism of unsaturated fatty acids in pike perch larvae (Sander lucioperca)
A1 Pérez Pérez, José Antonio
A1 Lund, Ivar
A1 Rodríguez, Covadonga
A1 Izquierdo, Maria S.
A1 El Kertaoui, Najlae
A1 Kestemont, Patrick
A1 Reis, Diana B.
A1 Dominguez, David
A2 Biología Animal y Edafología y Geología
A2 Grupo de investigación NUTRAHLIPIDS (Fisiología de los Lípidos y sus Derivados en la Nutrición Animal y Humana)
K1 Sander lucioperca
K1 Salinity
K1 HUFA
K1 Eicosanoids
K1 Enzymes
K1 Gene expression
K1 Metabolism
AB Combinations of nutritional requirements and husbandry rearing conditions during early ontogeny are poorlystudied in pikeperch (Sander lucioperca). The substitution of marine oils with vegetable oils has reduced stresstolerance and caused neurophysiological changes in pike perch larvae, but effects of environmental cues arelimited. Saline water influences on a range of physiological functions during early fish larval ontogeny and mayaffect FA metabolism, − elongation and desaturation - activity when given diets limited in LC PUFAs, but rich inshorter chain n-3 or n-6 PUFAs. Consequently, live Artemia differing in 18:2n-6 (LA) and 18:3n-3 (ALA) contentby enrichment with sunflower oil (SFO) or linseed oil (LO) were fed to 10 days post hatch (DPH) larvae andreared up to isosmotic salinities (0, 5, 10 ppt) until 30 DPH. Larval tissue FA composition was examined at 15, 25and 30 DPH. Besides, an in vivo assay was performed on 20 DPH larvae with 14C labelled FA including LA; ALA;20:4n-6 (ARA); 20:5n-3 (EPA) or 22:6n-3 (DHA) to establish FA incorporation and metabolism. At 30 DPH,performance, digestive enzymatic activity, eicosanoid activity, skeletal anomalies and stress sensitivity werefurther evaluated. Results on larval FA profiles suggest a low desaturation and elongation capability over LA andALA, with no significant effects of salinity or larval age on modulation of unsaturated fatty acid metabolism. Invivo assays revealed that regardless of salinity or diet, pikeperch possess a marked specificity to incorporate ARAand EPA compared to a poorer incorporation of DHA. Larvae exposed to a confinement stress test caused highacute mortality in all experimental groups except for a control group fed with Artemia enriched by a commercialDHA Selco emulsion. Growth performance was not significantly affected by salinity or dietary enrichment withSFO or LO, but influenced on larval enzymatic activity of pepsin, aminopeptidase, trypsin and alkaline phosphatase, while lipase activity was not significantly affected. Increased saline conditions significantly decreasedhormonal prostaglandin eicosanoid PGE2, PGE3 activity with the highest activity at 0 ppt. The prevalence ofsevere skeletal anomalies was generally high, affecting over 75% of the larval population with negative effectsby increase in salinity. The incidence of anomalies was higher on endochondral bones, namely maxillary, ranging from 58 to 83% of the population. These results agree well with those from expression of sox 9 and twist2genes; involved in chondrocyte ossification and differentiation.
YR 2019
FD 2019
LK http://riull.ull.es/xmlui/handle/915/35415
UL http://riull.ull.es/xmlui/handle/915/35415
LA en
NO https://doi.org/10.1016/j.aquaculture.2018.10.061
DS Repositorio institucional de la Universidad de La Laguna
RD 11-may-2024