RT info:eu-repo/semantics/article
T1 Polyunsaturated fatty acid metabolism in three fish species with different trophic level
A1 Pérez Pérez, José Antonio
A1 Galindo, A.
A1 Garrido, D.
A1 Monroig, Ó.
A1 Betancor, M.B.
A1 Acosta, N.G.
A1 Kabeya, N.
A1 Marrero, M.A.
A1 Bolaños, A.
A1 Rodríguez, C.
A2 Biología Animal y Edafología y Geología
A2 Grupo de investigación NUTRAHLIPIDS (Fisiología de los Lípidos y sus Derivados en la Alimentación Animal y Humana)
K1 Chelon labrosus
K1 Elovl5
K1 LC-PUFA
K1 Pegusa lascaris
K1 Sarpa salpa
AB Reducing the dependency of fishfeed for marine ingredients and species diversification are both consideredcrucial factors for the sustainable development of aquaculture. The substitution of fish oil (FO) by vegetable oils(VO) in aquafeeds is an economically feasible solution. However, such substitution may compromise the fishflesh content of essential n-3 long chain polyunsaturated fatty acids (n-3 LC-PUFA) and, therefore, its nutritionalvalue for human consumption. Likewise, there is a wide range of strategies to select new target species for sectordiversification, among which, the capacity to biosynthesize n-3 LC-PUFA from their C18 precursors abundant inVO might be considered as a fair preliminary strategy. Therefore, the aim of the present study was to analyze themetabolic fate of [1-14C] labeled 18:2n-6, 18:3n-3, 20:5n-3 and 22:6n-3 in isolated hepatocytes and enterocytesfrom wild individuals of three fish species with different trophic level: the marine herbivorous salema (Sarpasalpa), the strict carnivorous sand sole (Pegusa lascaris) and the omnivorous thicklip grey mullet (Chelon labrosus). These species were selected for their phylogenetic proximity to consolidated farmed species such asgilthead seabream (Sparus aurata), senegalese sole (Solea senegalensis), and golden grey mullet (Liza aurata),respectively. The study also assessed the molecular cloning, functional characterization and tissue distribution ofthe fatty acyl elongase (Elovl) gene, elovl5, involved in the biosynthetic metabolism of n-3 LC-PUFA. The threespecies were able to biosynthesize docosahexaenoic acid (22:6n-3). S. salpa seems to have similar biosyntheticcapacity than S. aurata, with a fatty acyl desaturase 2 (Fads2), with Δ6, Δ8 and Δ5 activities. P. lascaris showed awider Fads2 activity repertory than S. senegalensis, including Δ4 and residual Δ6/Δ5 activities. In C. labrosus,both Δ8 and Δ5 activities but not the Δ6 described for L. aurata were detected in the incubated cells. Elongationfrom C18 and C20 precursors to C20 and C22 products occurred in hepatocytes and enterocytes as well as in thefunctional characterization of Elovl5 by heterologous expression in yeast. Elovl5 showed a species specific expression pattern, with the highest rates observed in the liver, gut and brain in S. salpa and P. lascaris, and in thebrain for C. labrosus. In summary, the LC-PUFA biosynthesis capacity from S. salpa, P. lascaris and C. labrosusgreatly resembled that of their phylogenetic closer species. The three studied species could be further explored ascandidates for the aquaculture diversification from their potential ability to biosynthesize LC-PUFA.
YR 2021
FD 2021
LK http://riull.ull.es/xmlui/handle/915/35416
UL http://riull.ull.es/xmlui/handle/915/35416
LA en
NO https://doi.org/10.1016/j.aquaculture.2020.735761
DS Repositorio institucional de la Universidad de La Laguna
RD 10-may-2024