RT info:eu-repo/semantics/article
T1 Influence of dietary lipids and environmental salinity on the n-3 long-chain polyunsaturated fatty acids biosynthesis capacity of the marine teleost Solea senegalensis
A1 Pérez Pérez, José Antonio
A1 Marrero, Manuel
A1 Monroig, Óscar
A1 Betancor, Mónica
A1 Herrera, Marcelino
A1 Garrido, Diego
A1 Galindo, Ana
A1 Giráldez, Inmaculada
A1 Rodríguez, Covadonga
A2 Biología Animal y Edafología y Geología
A2 Grupo de investigación NUTRAHLIPIDS (Fisiología de los Lípidos y sus Derivados en la Alimentación Animal y Humana)
K1 Solea senegalensis
K1 Fads2
K1 Elovl5
K1 LC-PUFA biosynthesis
K1 diet
K1 salinity
K1 EPA
K1 DHA
AB Fish vary in their ability to biosynthesise long-chain polyunsaturated fatty acids (LC-PUFA)depending upon the complement and function of key enzymes commonly known as fatty acyldesaturases and elongases. It has been reported in Solea senegalensis the existence of a ∆4 desaturase,enabling the biosynthesis of docosahexaenoic acid (DHA) from eicosapentaenoic acid (EPA), whichcan be modulated by the diet. The present study aims to evaluate the combined effects of thepartial replacement of fish oil (FO) with vegetable oils and reduced environmental salinity in thefatty acid composition of relevant body compartments (muscle, hepatocytes and enterocytes), theenzymatic activity over α-linolenic acid (ALA) to form n-3 LC-PUFA through the incubation ofisolated hepatocytes and enterocytes with [1-14C] 18:3 n-3, and the regulation of the S. senegalensisfads2 and elovl5 in the liver and intestine. The presence of radiolabelled products, including 18:4n-3,20:4n-3 and EPA, provided compelling evidence that a complete pathway enabling the biosynthesisof EPA from ALA, establishing S. senegalensis, has at least one Fads2 with ∆6 activity. Dietarycomposition prevailed over salinity in regulating the expression of fads2, while salinity did so overdietary composition for elovl5. FO replacement enhanced the proportion of DHA in S. senegalensismuscle and the combination with 20 ppt salinity increased the amount of n-3 LC-PUFA in hepatocytes.
YR 2021
FD 2021
LK http://riull.ull.es/xmlui/handle/915/35418
UL http://riull.ull.es/xmlui/handle/915/35418
LA en
NO https://doi.org/10.3390/md19050254
DS Repositorio institucional de la Universidad de La Laguna
RD 27-sep-2024