RT info:eu-repo/semantics/article
T1  Esterification and modification of [1-14C] n-3 and n-6 polyunsaturated fatty acids in pikeperch (Sander lucioperca) larvae reared under linoleic or α-linolenic acid-based diets and variable environmental salinities
A1 Pérez Pérez, José Antonio
A1 Reis, D.B.
A1 Lund,  I.
A1 Acosta, N.G.
A1 Abdul-Jalbar, B.
A1 Bolaños, A.
A1 Rodríguez, C.
A2 Biología Animal y Edafología y Geología
A2 Grupo de investigación NUTRAHLIPIDS (Fisiología de los Lípidos y sus Derivados en la Alimentación Animal y Humana)
K1 Pikeperch larvae
K1 PUFA metabolism
K1 Phospholipids
K1 Dietary fatty acids
K1 Salinity
AB To elucidate the in vivo endogenous ability of pikeperch (Sander lucioperca) larvae to deacylate and reacylatephospholipids and to elongate and desaturate PUFAs, 20 days post hatch (DPH) fish were incubated with either[1-14C]20:4n-6 bound to PC and PE, or with free [1-14C]-labelled fatty acids (18:2n-6, 18:3n-3, 20:4n-6, 20:5n-3and 22:6n-3). The modulation capacity of both low LC-PUFAs but high 18C PUFAs precursors dietary supply andincreasing salinity on larval fatty acid metabolic pathways was also investigated. [1-14C]DHA was incorporatedinto larval tissues to a lower extent than [1-14C]ARA or [1-14C] EPA. [1-14C]ARA was significantly less abundantin larval tissues when provided bound to PE than when esterified into PC, indicating that PC is a better phospholipid source to provide LC-PUFA to pikeperch larvae. Radioactivity was mainly recovered into phospholipids,especially that of the three LC-PUFAs ARA, EPA and DHA. All substrates were primarily incorporated into PCexcept [1-14C]ARA which significantly did into PI. Both [1-14C]EPA and [1-14C]DHA showed a similar esterification pattern into lipid classes: PC > PE > PI > TAG, with [1-14C]DHA presenting the highest esterificationinto PE of all radiolabelled compounds (26.3% vs 3.6–14.2%). Although higher rearing salinities tended toincrease Δ6 desaturase activity, no radioactivity from [1-14C]18:2n-6 or [1-14C]18:3n-3 was detected in ARA orEPA, proving a deficiency of Δ5 activity and the inability of pikeperch to biosynthesize DHA. This work providesnovel information on the lipid metabolism of pikeperch at early development necessary for the design of liveprey enrichment protocols and dietary formulations adapted to larval metabolic capabilities.
YR 2020
FD 2020
LK http://riull.ull.es/xmlui/handle/915/35425
UL http://riull.ull.es/xmlui/handle/915/35425
LA en
NO https://doi.org/10.1016/j.cbpb.2020.110449
DS Repositorio institucional de la Universidad de La Laguna
RD 12-may-2024