RT info:eu-repo/semantics/article
T1 The lipid metabolism of Atlantic halibut (Hippoglossus hippoglossus, L.) larvae determined by 14C in vivo incubations
A1 Pérez Pérez, José Antonio
A1 Reis, Diana B.
A1 Hamre, Kristin
A1 Acosta, Nieves G.
A1 Norberg, Birgitta
A1 Harboe, Torstein
A1 Rodríguez, Covadonga
A2 Biología Animal y Edafología y Geología
A2 Grupo de investigación NUTRAHLIPIDS (Fisiología de los Lípidos y sus Derivados en la Alimentación Animal y Humana)
K1 Elongation/desaturation
K1 Esterification
K1 Fatty acids
K1 Hippoglossus hippoglossus larvae
K1 Lipid metabolism
AB The in vivo ability of Atlantic halibut (Hippoglossus hippoglossus) larvae to incorporate, de-acylate and re-acylate[1-14C]fatty acids (FA) into lipid classes and to elongate and desaturate those substrates was elucidated. To thispurpose, 30 days post-first-feeding (30 dpff) larvae were incubated in the presence of either free [1-14C]-FAs(18:2n-6, 18:3n-3, 20:4n-6, 20:5n-3 and 22:6n-3), [1-14C]18:1n-9 bound to mono- (MAG) and triacylglycerols(TAG), or [1-14C]20:4n-6 bound to phosphatidylcholine (PC) and phosphatidylethanolamine (PE). Halibut larvaecan efficiently incorporate dietary FAs through MAG, while FA incorporation when provided as free FAs andespecially bound to TAG or phospholipids seems to be very low. These results suggest that lipid digestion mightbe a limiting factor in the FA absorption and incorporation by 30 dpff Atlantic halibut. The present study alsoshows that esterification into phospholipids is a strong metabolic fate for dietary long-chain polyunsaturatedfatty acids (LC-PUFAs), and the capacity of 30 dpff Atlantic halibut larvae to metabolize dietary FAs throughelongation/desaturation processes. Nonetheless, neither EPA nor DHA were detected from [1-14C]ALA, indicatinga reduced capacity to biosynthesize these LC-PUFAs and the necessity for them to be adequately suppliedthrough the diet for proper larval development.
YR 2021
FD 2021
LK http://riull.ull.es/xmlui/handle/915/35427
UL http://riull.ull.es/xmlui/handle/915/35427
LA en
NO https://doi.org/10.1016/j.aquaculture.2021.736733
DS Repositorio institucional de la Universidad de La Laguna
RD 07-ago-2024