RT info:eu-repo/semantics/article
T1 Vitellogenin receptor and fatty acid profiles of individual lipid classes of oocytes from wild and captive-reared greater amberjack (Seriola dumerili) during the reproductive cycle
A1 Pérez Pérez, José Antonio
A1 Pousis, Chrysovalentinos
A1 Rodríguez, Covadonga
A1 De Ruvo, Pasquale
A1 De Virgilio, Caterina
A1 Mylonas, Constantinos C.
A1 Zupa, Rosa
A1 Passantino, Letizia
A1 Santamaria, Nicoletta
A1 Valentini, Luisa
A1 Corriero, Aldo
A2 Biología Animal y Edafología y Geología
A2 Grupo de investigación NUTRAHLIPIDS (Fisiología de los Lípidos y sus Derivados en la Nutrición Animal y Humana)
K1 Osteichthyes
K1 Mediterranean sea
K1 Oogenesis
K1 Vitellogenesis
K1 Reproduction
K1 Reproductive dysfunctions
K1 Phospholipids
AB The greater amberjack Seriola dumerili (Risso, 1810) is a large migratory pelagic fish occurring in tropical and temperate waters with a great potential for the world aquaculture industry. Previous studies showed that wild-caught female greater amberjack reared in sea cages and handled during the reproductive season, underwent extensive ovarian atresia. This atresia, however, was not related to an insufficient liver transcription or oocyte uptake of vitellogenin (Vtg). In the present study, the structure of two greater amberjack vitellogenin receptors, namely Vtgr (Lr8-) and Lrp13, was characterized. Moreover, vtgr and lrp13 gene expression and the fatty acid profiles of specific phospholipids and neutral lipids were compared in the ovaries of wild and captive-reared greater amberjack during different phases of the reproductive cycle (i.e. early gametogenesis, advanced gametogenesis and spawning). Ovarian vtgr and lrp13 transcription was more active during early gametogenesis, suggesting that vitellogenin receptor transcripts were synthesized by previtellogenic oocytes and remained in the cellular mRNA pool until oocytes resumed meiosis and entered into secondary growth (i.e. vitellogenesis). Rearing of wild-caught greater amberjack in captivity together with handling during the reproductive season was associated with a reduced vtgr and lrp13 transcription and with a diminished capacity of oocytes in the early phase of gametogenesis (primary oocyte growth) to enter into vitellogenesis. During early gametogenesis, remarkable differences in the fatty acid composition were observed between wild and captive-reared individuals: all phospholipids of captive fish displayed dramatic increases of saturates (16:0 and 18:0) and decreases of arachidonic acid (ARA) and docosahexaenoic acid (DHA). The present study confirms the susceptibility of greater amberjack reproductive function to handling stress and suggests that the consequent extensive atresia of vitellogenic follicles originated during the primary oocytes growth when the capacity of oocytes to synthesize vitellogenin receptors was reduced. The study also suggests that this reduced capacity was associated with an altered oocyte phospholipid fatty acid composition during early gametogenesis.
YR 2019
FD 2019
LK http://riull.ull.es/xmlui/handle/915/35434
UL http://riull.ull.es/xmlui/handle/915/35434
LA en
NO https://doi.org/10.1016/j.theriogenology.2019.08.014
DS Repositorio institucional de la Universidad de La Laguna
RD 07-ago-2024