RT info:eu-repo/semantics/article
T1 Functional diversifcation of teleost Fads2 fatty acyl desaturases occurs independently of the trophic level
A1 Pérez Pérez, José Antonio
A1 Garrido, Diego
A1 Kabeya, Naoki
A1 Betancor, Mónica B.
A1 Acosta, N. Guadalupe
A1 Tocher, Douglas R.
A1 Rodríguez, Covadonga
A1 Monroig, Óscar
K1 polyunsaturated fatty acid
AB The long-chain (≥C20) polyunsaturated fatty acid biosynthesis capacity of fsh varies among species,with trophic level hypothesised as a major factor. The biosynthesis capacity is largely dependent uponthe presence of functionally diversifed fatty acyl desaturase 2 (Fads2) enzymes, since many teleostshave lost the gene encoding a Δ5 desaturase (Fads1). The present study aimed to characterise Fads2from four teleosts occupying diferent trophic levels, namely Sarpa salpa, Chelon labrosus, Pegusalascaris and Atherina presbyter, which were selected based on available data on functions of Fads2from closely related species. Therefore, we had insight into the variability of Fads2 within the samephylogenetic group. Our results showed that Fads2 from S. salpa and C. labrosus were both Δ6desaturases with further Δ8 activity while P. lascaris and A. presbyter Fads2 showed Δ4 activity. Fads2activities of herbivorous S. salpa are consistent with those reported for carnivorous Sparidae species.The results suggested that trophic level might not directly drive diversifcation of teleost Fads2 asinitially hypothesised, and other factors such as the species’ phylogeny appeared to be more infuential.In agreement, Fads2 activities from P. lascaris and A. presbyter were similar to their correspondingphylogenetic counterparts Solea senegalensis and Chirostoma estor
YR 2019
FD 2019
LK http://riull.ull.es/xmlui/handle/915/35435
UL http://riull.ull.es/xmlui/handle/915/35435
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DS Repositorio institucional de la Universidad de La Laguna
RD 10-may-2024