RT info:eu-repo/semantics/article
T1 Iron and fur in the life cycle of the zoonotic pathogen vibrio vulnificus
A1 Sanjuan Caro, Eva
A1 Pajuelo, David
A1 Hernández-Cabanyero, Carla
A1 Chung-Te, Lee
A1 Silva-Hernández, Francisco Xavier
A1 Lien-I, Hor
A1 MacKenzie, Simon
A1 Amaro, Carmen
K1 Vibrio vulnificus
K1 Patógenos
K1 Microbiología
AB In this study, we aimed to analyze the global response to iron in the broad-range host pathogen Vibrio vulnificus under the hypothesis that iron is one of the main signals triggering survival mechanisms both inside and outside its hosts. To this end, we selected a strain from the main zoonotic clonal complex, obtained a mutant in the ferric-uptake regulator (Fur), and analyzed their transcriptomic profiles in both iron-excess and iron-poor conditions by using a strain-specific microarray platform. Among the genes differentially expressed, we identified around 250 as putatively involved in virulence and survival-related mechanisms. Then, we designed and performed a series of in vivo and in vitro tests to find out if the processes highlighted by the microarray experiments were in fact under iron and/or Fur control. Our results support the hypothesis that iron acts as a niche marker, not always through Fur, for V. vulnificus controlling its entire life cycle. This ranges from survival in the marine environment, including motility and chemotaxis, to survival in the blood of their hosts, including host-specific mechanisms of resistance to innate immunity. These mechanisms allow the bacterium to multiply and per sist inside and between their hosts.
YR 2016
FD 2016
LK http://riull.ull.es/xmlui/handle/915/35653
UL http://riull.ull.es/xmlui/handle/915/35653
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 19-oct-2024