RT info:eu-repo/semantics/article
T1 Eosinophil Subtypes in Adults with Asthma and Adults with Chronic Obstructive Pulmonary Disease
A1 Casanova Macario, Ciro
A1 Cabrera López, Carlos
A1 Sánchez Santos, Alejandra
A1 Lemes Castellano, Angelina
A1 Cazorla Rivero, Sara
A1 Breña Atienza, Joaquín
A1 González Dávila,  Enrique
A1 Celli, Bartolomé
A2 Grupo de Investigación sobre la enfermedad pulmonar obstructiva crónica (EPOC). Unidad de Investigación del Hospital Universitario La Candelaria.
K1 eosinophils
K1 subtypes
K1 asthma
K1 COPD
AB There is a differential response to eosinophilic modulation between patients with asthma and those with chronic obstructive pulmonary disease (COPD). There is also evidence of different subtypes of eosinophils in murine models. However, no study has compared eosinophil subtypes in individuals with COPD and in those with asthma. Objectives: Study the differences in eosinophils subtypes based in the surface protein expression in COPD patients and asthmatic patients. Methods: We studied 10 stable subjects in each of four groups: subjects with COPD, subjects with asthma, smokers without COPD, and healthy volunteers. Subjects with COPD and those with asthma were matched by age, sex, and FEV1% predicted. The following variables were determined: anthropometrics, smoking, exacerbation history, medication use, lung function, and comorbidities. Using flow cytometry and confocal microscopy from blood samples, we determined differences in eosinophil surface proteins and classified them as 1) resident eosinophils (Siglec-8+CD62L+IL-3Rlo) or 2) inflammatory eosinophils (iEos; Siglec-8+CD62LloIL-3Rhi). IL-5 receptor was also determined. Findings were validated in 59 patients with COPD and in 17 patients with asthma. Measurements and Main Results: Patients with asthma had a higher proportion of iEos (25 ± 15%) compared with those with COPD (0.5 ± 1%), smokers without COPD (0.14 ± 0.24%), and healthy volunteers (0.67 ± 1.72%). In patients with asthma, the proportion of iEos was independent of total eosinophil number. iEos had more IL-5 receptors than resident eosinophils (777.02 ± 124.55 vs. 598.35 ± 318.69; P < 0.01). In patients with COPD, there was no relation between iEos number and inhaled corticosteroid use, disease severity, or exacerbations rate. The findings in patients with COPD and those with asthma were confirmed in validation cohorts. Conclusions: There are differences in the subtypes of circulating eosinophils between patients with asthma and those with COPD. This could have clinical implications in the interpretation of eosinophil significance and the approach to therapy in these patients.
SN 1535-4970
YR 2023
FD 2023
LK http://riull.ull.es/xmlui/handle/915/35727
UL http://riull.ull.es/xmlui/handle/915/35727
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 16-may-2024