RT info:eu-repo/semantics/article
T1 Refractive index estimation in biological tissues by quantitative phase imaging
A1 Acebes Vindel, Ángel José
A1 Cairos, Carlos
A1 Oliva-García,  Ricardo
A1 Siverio,  Gabriela
A1 Trujillo-Sevilla,  Juan Manuel
A1 Rodríguez-Ramos, Jos´e Manuel
A2 Grupo de investigación: Mecanismos Moleculares en NeurodegeneraciónInstituto de tecnologías Biomédicas (ITB).
Grupo propio de la Universidad de La Laguna. IP del grupo: Ángel Acebes.
https://portalciencia.ull.es/investigadores/81920/detalle
K1 Microscopy
K1 Quantitative phase imaging (QPI)
K1 Refractive index
K1 Biological tissues
AB The refractive index (RI) of biological tissues represents a relevant optical property that correlates with intrinsicfactors such as dry mass or water content, providing valuable information about tissue composition. First, wereview available methods to measure the RI in biological tissues, making special emphasis on quantitative phaseimaging (QPI) and its ability to obtain RI maps as well as tri-dimensional projections of transparent samples atdiffraction-limited resolution. Second, we propose a simple, general QPI method to estimate the RI of complexmaterials without prior knowledge of sample thickness. Here we have tested several samples in various immersion media with different refractive indices (RIs) to use the lineal relation between the optical path difference(OPD) and the RI of the medium. Least squares regression operates to simultaneously determine the RI and thethickness with diffraction-limited resolution and nanometric transversal sensitivity. Our findings categorize a setof immersion media covering a good RI range, identify possible error sources and uncover the applicability of theQPI method, paving the way to its use on heterogeneous biological tissues
SN 
YR 2023
FD 2023
LK http://riull.ull.es/xmlui/handle/915/35814
UL http://riull.ull.es/xmlui/handle/915/35814
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 14-oct-2024