T1 Laurequinone, a lead compound against Leishmania
A1 López Arencibia, Atteneri
A1 García-Davis, Sara
A1 López-Arencibia, Atteneri
A1 Bethencourt-Estrella, Carlos J.
A1 San Nicolás-Hernández, Desirée
A1 Viveros-Valdez, Ezequiel
A1 Díaz-Marrero, Ana R.
A1 Fernández, José J.
A1 Piñero, José E.
A1 Lorenzo Morales, Jacob
K1 laurequinone
K1 sesquiterpene
K1 Laurencia
K1 Leishmania amazonensis
K1 leishmaniasis
AB Among neglected tropical diseases, leishmaniasis is one of the leading causes, not onlyof deaths but also of disability-adjusted life years. This disease, caused by protozoan parasites ofthe genus Leishmania, triggers different clinical manifestations, with cutaneous, mucocutaneous, andvisceral forms. As existing treatments for this parasitosis are not sufficiently effective or safe for thepatient, in this work, different sesquiterpenes isolated from the red alga Laurencia johnstonii havebeen studied for this purpose. The different compounds were tested in vitro against the promastigoteand amastigote forms of Leishmania amazonensis. Different assays were also performed, includingthe measurement of mitochondrial potential, determination of ROS accumulation, and chromatincondensation, among others, focused on the detection of the cell death process known in this type oforganism as apoptosis-like. Five compounds were identified that displayed leishmanicidal activity:laurequinone, laurinterol, debromolaurinterol, isolaurinterol, and aplysin, showing IC50 valuesagainst promastigotes of 1.87, 34.45, 12.48, 10.09, and 54.13 µM, respectively. Laurequinone was themost potent compound tested and was shown to be more effective than the reference drug miltefosineagainst promastigotes. Different death mechanism studies carried out showed that laurequinoneappears to induce programmed cell death or apoptosis in the parasite studied. The obtained resultsunderline the potential of this sesquiterpene as a novel anti-kinetoplastid therapeutic agent
SN 1660-3397
YR 2023
FD 2023
LK http://riull.ull.es/xmlui/handle/915/35827
UL http://riull.ull.es/xmlui/handle/915/35827
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 24-nov-2024