RT info:eu-repo/semantics/article
T1 Activity and mechanism of the action of zeylasterone against Bacillus subtilis.
A1 León Guerra, Leandro Jesús
K1 antimicrobial activity
K1 Bacillus subtilis
K1 cytoplasmic membrane
K1 macromolecular synthesis
K1 6-oxophenolic triterpenoids
AB Aims: To investigate the antimicrobial properties of 6-oxophenolic triterpenoids isolated from Maytenus blepharodes against different micro-organisms and the mode of action on Bacillus subtilis. Methods and Results: The activity of zeylasterone and demethylzeylasterone was evaluated by microdilution method. Zeylasterone showed a higher activity, being active against Gram-positive bacteria (minimum inhibitory concentration 3-20 μg ml-1) and Candida albicans (10 μg ml-1). Killing curves revealed a bacteriostatic effect on B. subtilis that was dependent on the growth phase and inoculum size. Zeylasterone caused cell membrane alterations in B. subtilis, as shown by potassium leakage and formation of mesosome-like structures. However, membrane disruption was not revealed by either LIVE/DEAD Baclight assay or measurement of intracellular constituent efflux. Zeylasterone showed an early effect on N-acetyl-glucosamine and uridine incorporation and later on that of thymidine and leucine. Conclusions: Diverse micro-organisms exhibit sensitivities towards compounds studied. The permeability changes in the cytoplasmic membrane and nonsimultaneous ceasing of macromolecular synthesis suggest that zeylasterone could act on multiple targets on B. subtilis. Significance and Impact of the Study: The activity showed against B. subtilis as a model of spore-forming bacteria would provide valuable information for further studies in the development of 6-oxophenolic triterpenoids as antiseptic and disinfectant properties. © 2007 The Authors.
SN 1364-5072
YR 2008
FD 2008
LK http://riull.ull.es/xmlui/handle/915/35864
UL http://riull.ull.es/xmlui/handle/915/35864
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 19-may-2024