RT info:eu-repo/semantics/article
T1 Detection of Clavibacter michiganensis subsp. michiganensis in tomato seeds using immunomagnetic separation
A1 León Guerra, Leandro Jesús
A1 Siverio, F.
A1 Rodríguez, A.
K1 Clavibacter michiganensis subsp. michiganensis
K1 Detection
K1 Bacterial canker
K1 Tomato seeds
K1 Immunomagnetic separation
K1 IMS
AB The use of pathogen-free plant material is the main strategy for controlling bacterial canker of tomato caused by Clavibactermichiganensis subsp. michiganensis. However, detection and isolation of this pathogen from seeds before field or greenhousecultivation is difficult when the bacterium is at low concentration and associated microbiota are present. Immunomagneticseparation (IMS), based on the use of immunomagnetic beads (IMBs) coated with specific antibodies, was used to capture C.michiganensis subsp. michiganensis cells, allowing removal of non-target bacteria from samples before plating on non-selectivemedium. Different concentrations of IMBs and of two antisera were tested, showing that IMS with 106IMBs/ml coated with apolyclonal antiserum at 1/3200 dilution recovered more than 50% of target cells from initial inocula of 103 to 100CFU/ml.Threshold detection was lower than 10CFU/ml even in seed extracts containing seed debris and high populations of non-targetbacteria. The IMS permitted C. michiganensis subsp. michiganensis isolation from naturally infected seeds with higher sensitivityand faster than direct isolation on the semiselective medium currently used and could become a simple viable system for routinelytesting tomato seed lots in phytosanitary diagnostic laboratories.© 2006 Elsevier B.V. All rights reserved.
YR 2066
FD 2066
LK http://riull.ull.es/xmlui/handle/915/35867
UL http://riull.ull.es/xmlui/handle/915/35867
LA en
NO DOI: 10.1016/J.MIMET.2006.03.007
DS Repositorio institucional de la Universidad de La Laguna
RD 11-may-2024