RT info:eu-repo/semantics/article
T1 Bioguided Isolation of Active Compounds from Rhamnus alaternus against Methicillin-Resistant Staphylococcus aureus (MRSA) and Panton-Valentine Leucocidin Positive Strains (MSSA-PVL)
A1 Zeouk, Ikrame
A1 Ouedrhiri, Wessal
A1 Sifaoui, Ines
A1 López Bazzocchi, Isabel
A1 Piñero Barroso, José Enrique
A1 Jiménez Díaz, Ignacio Antonio
A1 Lorenzo Morales, Jacob
A1 Bekhti, Khadija
K1 ethnobotany
K1 bioguided fractionation
K1 MRSA
K1 MSSA-PVL
K1 anti-staphylococcal activity
K1 Rhamnus alaternus
K1 emodin
AB Despite intensified efforts to develop an effective antibiotic, S. aureus is still a major cause ofmortality and morbidity worldwide. The multidrug resistance of bacteria has considerably increasedthe difficulties of scientific research and the concomitant emergence of resistance is to be expected. Inthis study we have investigated the in vitro activity of 15 ethanol extracts prepared from Moroccanmedicinal plants traditionally used for treatment of skin infections. Among the tested species I. viscosa,C. oxyacantha, R. tinctorum, A. herba alba, and B. hispanica showed moderate anti-staphylococcal activity.However, R. alaternus showed promising growth-inhibitory effects against specific pathogenic bacteriaespecially methicillin-susceptible Staphylococcus aureus Panton-Valentine leucocidin positive (MSSAPVL)and methicillin-resistant S. aureus (MRSA). The bioguided fractionation of this plant usingsuccessive chromatographic separations followed by nuclear magnetic resonance (NMR) and massspectrometry (MS) including EIMS and HREIMS analysis yielded the emodin (1) and kaempferol(2). Emodin being the most active with MICs ranging between 15.62 and 1.95  g/mL and showinghigher activity against the tested strains in comparison with the crude extract, its mechanism ofaction and the structure-activity relationship were interestingly discussed. The active compound hasnot displayed toxicity toward murine macrophage cells. The results obtained in the current studysupport the traditional uses of R. alaternus and suggest that this species could be a good source forthe development of new anti-staphylococcal agents.
YR 2021
FD 2021
LK http://riull.ull.es/xmlui/handle/915/35932
UL http://riull.ull.es/xmlui/handle/915/35932
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 27-nov-2024