RT info:eu-repo/semantics/article
T1 High diversity of Bradyrhizobial species fix nitrogen with woody legume Spartocytisus supranubius in a high mountain ecosystem.
A1 Díaz Peña, Francisco Javier
A1 Pulido Suárez, Laura
A1 Notario del Pino, Jesús
A1 Perdomo González, Adolfo
A1 Ggonzález Rodríguez, Águeda M.
A1 León Barrios, Milagros
K1 Bradyrhizobia
K1 Legume (Genisteae)
K1 Rhizobia
K1 Symbiosis symbiovars
K1 High mountain soils
AB The symbiosis between rhizobia and legumes is of pivotal importance in nitrogen-poor ecosystems. Furthermore, as it is a specific process (most legumes only establish a symbiosis with certain rhizobia), it is of great interest to know which rhizobia are able to nodulate key legumes in aspecific habitat. This study describes the diversity of the rhizobia that are able to nodulate the shrublegume Spartocytisus supranubius in the harsh environmental conditions of the high mountain ecosystem of Teide National Park (Tenerife). The diversity of microsymbionts nodulating S. supranubius was estimated from a phylogenetic analysis of root nodule bacteria isolated from soils at three selected locations in the park. The results showed that a high diversity of species of Bradyrhizobium and two symbiovars can nodulate this legume. Phylogenies of ribosomal and housekeeping genes showed these strains distributed into three main clusters and a few isolates on separate branches. These clusters consist of strains representing three new phylogenetic lineages of the genus Bradyrhizobium. Two of these lineages belong to the B. japonicum superclade, which we refer to as B. canariense-like and B. hipponense-like, as the type strains of these species are the closest species to our isolates. The third main group was clustered within the B. elkanii superclade and is referred to as B. algeriense-like as B. algeriense is its closest species. This is the first time that bradyrhizobia of the B. elkanii superclade have been reported for the canarian genista. Furthermore, our results suggest that these three main groups might belong to potential new species of the genus Bradyrhizobium. Analysis of the soilphysicochemical properties of the three study sites showed some significant differences in severalparameters, which, however, did not have a major influence on the distribution of bradyrhizobialgenotypes at the different locations. The B. algeriense-like group had a more restrictive distribution pattern, while the other two lineages were detected in all of the soils. This suggests that the microsymbionts are well adapted to the harsh environmental conditions of Teide National Park.
YR 2023
FD 2023
LK http://riull.ull.es/xmlui/handle/915/35955
UL http://riull.ull.es/xmlui/handle/915/35955
LA en
NO https://doi.org/10.3390/microorganisms11051244
DS Repositorio institucional de la Universidad de La Laguna
RD 18-may-2024