RT info:eu-repo/semantics/article
T1 Exploring the Anti-Infective Value of Inuloxin A Isolated from Inula viscosa against the Brain-Eating Amoeba (Naegleria fowleri) by Activation of Programmed Cell Death
A1 Zeouk, Ikrame
A1 Sifaoui, Ines
A1 Rizo Liendo, Aitor
A1 Arberas Jiménez, Iñigo
A1 Reyes Batlle, María
A1 López Bazzocchi, Isabel
A1 Bekhti, Khadija
A1 Piñero Barroso, José Enrique
A1 Jiménez Díaz, Ignacio Antonio
A1 Lorenzo Morales, Jacob
K1 Inula viscosa
K1 bioguided fractionation
K1 inuloxin A
K1 brain-eating amoeba
K1 Naegleria fowleri
K1 programmed cell death
AB Primary amoebic meningoencephalitis (PAM), caused by the pathogenicfree-living amoeba Naegleria fowleri, is a rare but fatal disease. Nowadays, no fully effectivetherapy is available to erradicate or prevent this disease. Natural products could constitute apromising source of useful bioactive compounds in drug discovery. The present study is acharacterization of main active compounds from the ethanolic extract of Inula viscosa(Asteraceae) leaves against N. fowleri trophozoites. Four compounds (1−4) weresuccessfully identified by spectroscopic techniques, but only inuloxin A displayed apotential antiamoebic activity with an IC50 of 21.27 μM. The specificity of this compoundtoward the studied strain leads us to analyze the insight into its mechanism of action byperforming in vitro assays of programmed cell death markers and to discuss the structure−activity relationship (SAR). The obtained results demonstrated that inuloxin A interfereswith various processes leading to membrane damage, mitochondria alteration, chromatincondensation, and ROS accumulation, which highlight features specific to apoptosis. Thecurrent findings could be a promising step for developing new effective drugs against PAM.
YR 2021
FD 2021
LK http://riull.ull.es/xmlui/handle/915/36034
UL http://riull.ull.es/xmlui/handle/915/36034
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 18-nov-2024