RT info:eu-repo/semantics/article
T1 Development and Validation of an HPLC-PDA Method for Biologically Active Quinonemethide Triterpenoids Isolated from Maytenus chiapensis
A1 Taddeo, Vito Alessandro
A1 Guardado Castillo, Ulises
A1 Martínez, Morena Lizette
A1 Menjivar, Jenny
A1 Jiménez Díaz, Ignacio Antonio
A1 Núñez, Marvin José
A1 López Bazzocchi, Isabel
K1 Maytenus chiapensis
K1 Celastraceae
K1 quinonemethide triterpenoids
K1 pristimerin
K1 tingenone
K1 HPLC-PDA
AB Background: Quinonemethide triterpenoids, known as celastroloids, constitute a relativelysmall group of biologically active compounds restricted to the Celastraceae family and, therefore,they are chemotaxonomic markers for this family. Among this particular type of metabolite,pristimerin and tingenone are considered traditional medicines in Latin America. The aim of thisstudy was the isolation of the most abundant celastroloids from the root bark of Maytenus chiapensis,and thereafter, to develop an analytical method to identify pristimerin and tingenone in theCelastraceae species. Methods: Pristimerin and tingenone were isolated from the n-hexane-Et2Oextract of the root bark of M. chiapensis through chromatographic techniques, and were used asinternal standards. Application of a validated RP HPLC-PDA method was developed for thesimultaneous quantification of these two metabolites in three different extracts, n-hexane-Et2O,methanol, and water, to determine the best extractor solvent. Results: Concentration values showedgreat variation between the solvents used for extraction, with the n-hexane–Et2O extract being therichest in pristimerin and tingenone. Conclusions: M. chiapensis is a source of two biologicallyactive quinonemethide triterpenoids. An analytical method was developed for the qualification andquantification of these two celastroloids in the root bark extracts of M. chiapensis. The validatedmethod reported herein could be extended and be useful in analyzing Celastraceae species and realcommercial samples.
YR 2019
FD 2019
LK http://riull.ull.es/xmlui/handle/915/36067
UL http://riull.ull.es/xmlui/handle/915/36067
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 22-nov-2024