RT info:eu-repo/semantics/article
T1 Antioxidant and Leishmanicidal Evaluation of Pulicaria Inuloides Root Extracts: A Bioguided Fractionation
A1 Fadel, Hamza
A1 Sifaoui, Ines
A1 López Arencibia, Atteneri
A1 Reyes Batlle, María
A1 Jiménez Díaz, Ignacio Antonio
A1 Lorenzo Morales, Jacob
A1 Ghedadba, Nabil
A1 Benayache, Samir
A1 Piñero Barroso, José Enrique
A1 López Bazzocchi, Isabel
K1 Leishmania
K1 antioxidant
K1 Pulicaria
K1 bioguided
K1 therapy
AB Leishmaniasis remains a major world health problem, and in particular, Algeria rankssecond for the incidence of cutaneous leishmaniasis. Pulicaria inuloides is a well-known ArabianPeninsula medicinal plant. In the present study, the chloroform, ethyl acetate and n-butanol extractsfrom the roots of Pulicaria inuloides were analyzed for antioxidant activity and its correlation withthe total phenolic and flavonoid contents. The highest antioxidant activity using a DPPH assay wasshowed by the ethyl acetate extract (IC50 4.08  g/mL), which also had the highest total phenoliccontent (307.12  gAGE). Furthermore, P. inuloides root extracts were evaluated against Leishmaniaamazonensis and Leishmania donovani. The results highlighted the chloroform extract as the most activeone against both tested Leishmania strains. A bioguided fractionation of the chloroform extract led tothe isolation of (8R:8S)-(75:25 er)-10-isobutyryloxy-8,9-epoxy-thymol isobutyrate as the main bioactivecomponent, showing a potent leishmanicidal activity on L. amazonensis promatigote and amastigotestages (IC50 5.03 and 2.87  M, respectively) and a good selectivity index on murine macrophages(CC50 19.37  M). This study provides the first report of the antioxidant and leishmanicidal activitiesof P. inuloides root extracts and the results point to this species as a source of potential bioactive agents
YR 2019
FD 2019
LK http://riull.ull.es/xmlui/handle/915/36070
UL http://riull.ull.es/xmlui/handle/915/36070
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DS Repositorio institucional de la Universidad de La Laguna
RD 26-dic-2024