RT info:eu-repo/semantics/article
T1 Combined sustained release of BMP2 and MMP10 accelerates bone formation and mineralization of calvaria critical size defect in mice.
A1 Reyes Rodríguez, Ricardo
A1 Rodríguez, Jose Antonio
A1 Orbe, Josune
A1 Arnau,  María Rosa
A1 Évora García, Carmen María
A1 Delgado Hernández, Araceli Rita
K1 BMP-2
K1 MMP10
K1 Sustained
K1 Release
K1 Bone repair
K1 Mineralization
K1 Histomorphometry
AB The effect of dual delivery of bone morphogenetic protein-2 (BMP-2) and matrix metalloproteinase 10 (MMP10) on bone regeneration was investigated in a murine model of calvarial critical-size defect,hypothesizing that it would result in an enhanced bone formation. Critical-size calvarial defects (4 mm diameter) were created in mice and PLGA microspheres preloaded with either BMP-2, MMP10 or a microsphere combination of both were transplanted into defect sites at different doses. Empty microspheres were used as the negative control. Encapsulation efficiency was assessed and in vivo releasekinetics of BMP-2 and MMP10 were examined over 14 days. Histological analyses were used to analyze bone formation after four and eight weeks. Combination with MMP10 (30 ng) significantly enhanced BMP-2 (600 ng)-mediated osteogenesis, as confirmed by the increase in percentage of bone fill (p < .05) at four weeks. Moreover, it also increased mineral apposition rate (p < .05), measured by double labeling with tetracycline and calceine. MMP10 accelerates bone repair by enhancing BMP-2-promoted bone healing and improving the mineralization rate. In conclusion combination of MMP10 and BMP-2 may become a promising strategy for repair and regeneration of bone defects.
SN 1071-7544 (Print)
YR 2018
FD 2018
LK http://riull.ull.es/xmlui/handle/915/36127
UL http://riull.ull.es/xmlui/handle/915/36127
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 06-oct-2024