RT info:eu-repo/semantics/article
T1 Identification of new genetic clusters in glioblastoma multiforme: EGFR status and ADD3 losses Influence prognosis.
A1 Navarro Cerveró, Lara
A1 San-Miguel, Teresa
A1 Megías Vericat, Javier
A1 Santonja, Nuria
A1 Calabuig, Silvia
A1 Muñoz-Hidalgo, Lisandra
A1 Roldán, Pedro
A1 Cerdá-Nicolás, Miguel
A1 López-Ginés, Concha
K1 glioblastoma
K1 IDH
K1 ADD3
K1 EGFR
K1 survival
K1 high throughout techniques
K1 precision
AB Glioblastoma multiforme (GB) is one of the most aggressive tumors. Despite continuous efforts to improve its clinical management, there is still no strategy to avoid a rapid and fatal outcome. EGFR amplification is the most characteristic alteration of these tumors. Although effective therapy against it has not yet been found in GB, it may be central to classifying patients. We investigated somatic-copy number alterations (SCNA) by multiplex ligation-dependent probe amplification in a series of 137 GB, together with the detection of EGFRvIII and FISH analysis for EGFR amplification. Publicly available data from 604 patients were used as a validation cohort. We found statistical associations between EGFR amplification and/or EGFRvIII, and SCNA in CDKN2A, MSH6, MTAP and ADD3. Interestingly, we found that both EGFRvIII and losses on ADD3 were independent markers of bad prognosis (p = 0.028 and 0.014, respectively). Finally, we got an unsupervised hierarchical classification that differentiated three clusters of patients based on their genetic alterations. It offered a landscape of EGFR co-alterations that may improve the comprehension of the mechanisms underlying GB aggressiveness. Our findings can help in defining different genetic profiles, which is necessary to develop new and different approaches in the management of our patients.
SN 2073-4409
YR 2020
FD 2020
LK http://riull.ull.es/xmlui/handle/915/36170
UL http://riull.ull.es/xmlui/handle/915/36170
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 05-jul-2024