RT info:eu-repo/semantics/masterThesis
T1 Estudio del efecto de la proteína no estructural 5 del virus Zika en la producción de IFN de tipo I
A1 Hernández Cabrera, Raúl
A2 Máster Univ. en Investigación y Diagnóstico de Enfermedades Tropicales
AB The Zika virus (ZIKV) differs from other flaviviruses due to its neurotropism and neurotoxicity,which are associated with the most deleterious effects of the infection, such as CongenitalZika Syndrome (CZS) and Guillain-Barré Syndrome. Both the blood-brain barrier and theplacental barrier seem to be disrupted, and the ability of the nonstructural protein 5 (NS5) tointerfere with the innate immune response, especially in the production of type I interferon(IFN-I), is crucial in this process. Here, we demonstrate the impact of the ZIKV NS5 viralprotein on the innate immune response in HEK-Lucia cells, a reporter cellular model for IFN-Iexpression. We discovered that promoting post-translational modifications (PTMs) such asubiquitination, SUMOylation, or blocking autophagy abolishes NS5 ability to inhibit IFN-I. Werevealed that Lysine 330 of NS5 directs its capacity to subvert IFN-I expression in responseto infection. Our findings demonstrate that PTMs are critical in NS5 suppression of IFN-I. ZIKVutilizes the cellular ubiquitination machinery to facilitate evasion of the immune response.Given the global impact of ZIKV and its potential for a pandemic, understanding this virusbetter is crucial for developing prevention, control, and potential treatment strategies.
YR 2024
FD 2024
LK http://riull.ull.es/xmlui/handle/915/36523
UL http://riull.ull.es/xmlui/handle/915/36523
LA es
DS Repositorio institucional de la Universidad de La Laguna
RD 11-jul-2024