RT info:eu-repo/semantics/article
T1 Early Transcriptional Liver Signatures in Experimental Visceral Leishmaniasis
A1 Palacios, Génesis
A1 Diaz Solano, Raquel
A1 Valladares Hernández, Basilio
A1 Dorta Guerra, Roberto
A1 Carmelo, Emma
A2 Obstetricia y GinecologíaPediatría, Medicina Preventiva y Salud Pública, Toxicología y Medicina Legal y Forense y Parasitología
A2 Investigación en Parasitología
K1 transcriptional profiling
K1 high-throughput RT-qPCR
K1 multivariate analysis
K1 principal component analysis
K1 Leishmania infantum
K1 gene expression
K1 gene signature
K1 liver
K1 mouse experimental model
AB Transcriptional analysis of complex biological scenarios has been used extensively, even though sometimes the results of such analysis may prove imprecise or difficult to interpret due to an overwhelming amount of information. In this study, a large-scale real-time qPCR experiment was coupled to multivariate statistical analysis in order to describe the main immunological events underlying the early L. infantum infection in livers of BALB/c mice. High-throughput qPCR was used to evaluate the expression of 223 genes related to immunological response and metabolism 1, 3, 5, and 10 days post infection. This integrative analysis showed strikingly different gene signatures at 1 and 10 days post infection, revealing the progression of infection in the experimental model based on the upregulation of particular immunological response patterns and mediators. The gene signature 1 day post infection was not only characterized by the upregulation of mediators involved in interferon signaling and cell chemotaxis, but also the upregulation of some inhibitory markers. In contrast, at 10 days post infection, the upregulation of many inflammatory and Th1 markers characterized a more defined gene signature with the upregulation of mediators in the IL-12 signaling pathway. Our results reveal a significant connection between the expression of innate immune response and metabolic and inhibitory markers in early L. infantum infection of the liver.
YR 2021
FD 2021
LK http://riull.ull.es/xmlui/handle/915/37291
UL http://riull.ull.es/xmlui/handle/915/37291
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 12-sep-2024