RT info:eu-repo/semantics/article
T1 Striatal astrocytes engulf dopaminergic debris in Parkinson's disease: A study in an animal model.
A1 Morales Pérez, Ingrid
A1 Sánchez, Alberto
A1 Rodríguez Sabaté, Clara
A1 Rodríguez, Manuel
A2 Ciencias Médicas Básicas
A2 Grupo de Neurobiología y Neurología Experimental
AB The role of astrocytes in Parkinson’s disease is still not well understood. This work studiedthe astrocytic response to the dopaminergic denervation. Rats were injected in the lateralventricles with 6-hydroxydopamine (25μg), inducing a dopaminergic denervation of the striatum not accompanied by non-selective tissue damage. The dopaminergic debris were foundwithin spheroids (free-spheroids) which retained some proteins of dopaminergic neurons(e.g., tyrosine hydroxylase, the dopamine transporter protein, and APP) but not others (e.g.,α-synuclein). Free-spheroids showed the initial (LC3-autophagosomes) but not the late(Lamp1/Lamp2-lysosomes) components of autophagy (incomplete autophagy), preparingtheir autophagosomes for an external phagocytosis (accumulation of phosphatidylserine).Free-spheroids were penetrated by astrocyte processes (fenestrated-spheroids) whichmade them immunoreactive for GFAP and S100β, and which had some elements needed tocontinue the debris degradation (Lamp1/Lamp2). Finally, proteins normally found in neurons(TH, DAT and α-synuclein) were observed within astrocytes 2–5 days after the dopaminergic degeneration, suggesting that the intracellular contents of degenerated cells had beentransferred to astrocytes. Taken together, present data suggest phagocytosis as a physiological role of striatal astrocytes, a role which could be critical for cleaning striatal debris during the initial stages of Parkinson’s disease.
YR 2017
FD 2017
LK http://riull.ull.es/xmlui/handle/915/38568
UL http://riull.ull.es/xmlui/handle/915/38568
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DS Repositorio institucional de la Universidad de La Laguna
RD 22-dic-2024