RT info:eu-repo/semantics/article
T1 The degeneration of dopaminergic synapses in Parkinson's disease: A selective animal model.
A1 Morales Pérez, Ingrid
A1 Sánchez, Alberto
A1 Rodríguez Sabaté, Clara
A1 Rodríguez, Manuel
A2 Ciencias Médicas Básicas
A2 Grupo de Neurobiología y Neurología Experimental
K1 Parkinson’s disease
K1 Dying-back degeneration
K1 Astrocyte
K1 Microglia
K1 Striatum
K1 6OHDA
K1 Lateral ventricle
AB Available evidence increasingly suggests that the degeneration of dopamine neurons in Parkinson’s disease starts in the striatal axons and synaptic terminals. A selective procedure is described here to study the mechanisms involved in the striatal denervation of dopaminergic terminals. This procedure can also be used to analyze mechanisms involved in the dopaminergic re-innervation of the striatum, and the role of astrocytes and microglia in both processes. Adult Sprague-Dawley rats were injected in the lateral ventricles with increasing doses of 6-hydroxydopamine (12–50 g), which generated a dose-dependent loss of dopaminergic synapses and axons in the striatum, followed by an axonal sprouting (weeks later) and by a progressive recovery of striatal dopaminergic synapses (months later). Both the degeneration and regeneration of the dopaminergic terminals were accompanied by astrogliosis. Because the experimental manipulations did not induce unspecific damage in the striatal tissue, this method could be particularly suitable to study the basic mechanisms involved in the distal degeneration and regeneration of dopaminergic nigrostriatal neurons, and the possible role of astrocytes and microglia in the dynamics of both processes.
YR 2015
FD 2015
LK http://riull.ull.es/xmlui/handle/915/38596
UL http://riull.ull.es/xmlui/handle/915/38596
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 06-oct-2024