RT info:eu-repo/semantics/article
T1 Relationship between mineralization of synthetic lignins and the generation of hydroxyl radicals by laccase and a low molecular weight substance produced by Petriellidium fusoideum.
A1 Hernández Fernaud, Juan Ramón
A1 Gonzales, L.
A1 Perestelo, Fernando R.
A1 Carnicero, A.
A1 Falcón, M.A.
A2 BioquímicaMicrobiología, Biología Celular y Genética
A2 Ärea de microbiología de la Universidad de La Laguna
AB A new ligninolytic fungal strain was isolated from the Peruvian Amazon forest. Its enzymatic profile revealed a laccase as the only enzyme related to lignin metabolism. Its partial inhibition with thioglycolate also reduces mineralization. Production of hydroxyl radicals was detected from the first day of incubation. Their inhibition involves a substantial decrease in the mineralization of synthetic lignins. Besides this, the fungus produced a protein-free low molecular weight substance “LMW-S” (about 1.6 kDa) containing 0.1% Fe2 .To elucidate the cooperation between laccase and the LMW-S in producing the hydroxyl radicals (.OH) excreted by this fungus in lignin degradation, different systems composed of partially purified enzyme, LMW-S, and some substrates recognised by the laccase were assayed. A hypothetical mechanism is proposed for the generation of .OH radical in this system, in an attempt to explain its participation in the mineralization produced by the fungus.
YR 2002
FD 2002
LK http://riull.ull.es/xmlui/handle/915/38812
UL http://riull.ull.es/xmlui/handle/915/38812
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DS Repositorio institucional de la Universidad de La Laguna
RD 24-nov-2024