RT info:eu-repo/semantics/article
T1 Chromogranins A and B are key proteins in amine accumulation, but the catecholamine secretory pathway is conserved without them.
A1 Hernández Fernaud, Juan Ramón
A1 Díaz Vera, Jésica
A1 Camacho, Marcial
A1 Machado Ponce, José David
A1 Domínguez, Natalia
A1 Montesinos, Mónica S.
A1 Luján, Rafael
A1 Borges Jurado, Ricardo
A2 BioquímicaMicrobiología, Biología Celular y Genética
A2 Unidad de FarmacologíaFacultad de Medicina, Universidad de La Laguna,
K1 adrenal
K1 amperometry
K1 chromaffin cells
AB Chromogranins are the main soluble proteins in the large dense core secretory vesicles (LDCVs) found in aminergic neurons and chromaffin cells. We recently demonstrated that chromogranins A and B each regulate the concentration of adrenaline in chromaffin granules and its exocytosis. Here we have further studied the role played by these proteins by generating mice lacking both chromogranins. Surprisingly, these animals are both viable and fertile. Although chromogranins are thought to be essential for their biogenesis, LDCVs were evident in these mice. These vesicles do have a somewhat atypical appearance and larger size. Despite their increased size, single-cell amperometry recordings from chromaffin cells showed that the amine content in these vesicles is reduced by half. These data demonstrate that although chromogranins regulate the amine concentration in LDCVs, they are not completely essential, and other proteins unrelated to neurosecretion, such as fibrinogen, might compensate for their loss to ensure that vesicles are generated and the secretory pathway conserved.—DíazVera, J., Camacho, M., Machado, J. D., Domínguez, N., Montesinos, M. S., Herna ´ndez-Fernaud, J. R., Luja ´n, R., Borges, R. Chromogranins A and B are key proteins in amine accumulation, but the catecholamine secretory pathway is conserved without them.
YR 2012
FD 2012
LK http://riull.ull.es/xmlui/handle/915/38819
UL http://riull.ull.es/xmlui/handle/915/38819
LA en
NO DOI: 10.1096/fj.11-181941
DS Repositorio institucional de la Universidad de La Laguna
RD 24-nov-2024