RT info:eu-repo/semantics/article
T1 RAPD method useful for distinguishing Leishmania species: design of specific primers for L. braziliensis.
A1 Ortega Rivas, Antonio
A1 Martínez Carretero, Enrique
A1 Alonso, V.
A1 Quispe, A.
A1 Thomas, M.C .
A1 Alonso, R.
A1 Piñero Barroso, José Enrique
A1 González, A.C.
A1 Valladares Hernández, Basilio
A2 Didácticas Específicas
A2 Instituto de Enfermedades Tropicales y Salud Pública de Canarias
K1 RAPD
K1 PCR
K1 primer
K1 Leishmania braziliensis
AB The technique of Random Amplification Polymorphic DNA allows fragments of the genome to be amplified by means of polymerase chain reaction (PCR) without previous knowledge of their sequences. The protozoa of the genus Leishmania present great genetic variability, making it difficult to characterize the different species. A method is developed with a single 10-mers long primer, which allows the species L. braziliensis, L. mexicana, L. infantum, L. tropica, L. chagasi, L. amazonensis and L. major to be differentiated. These products amplified by RAPD have also facilitated the design of some primers that amplify L. braziliensis DNA exclusively.
YR 2003
FD 2003
LK http://riull.ull.es/xmlui/handle/915/38839
UL http://riull.ull.es/xmlui/handle/915/38839
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DS Repositorio institucional de la Universidad de La Laguna
RD 16-oct-2024