RT info:eu-repo/semantics/article
T1 The Nucleocapsid protein triggers the main humoral immune response in COVID-19 patients
A1 Hernández Fernaud, Juan Ramón
A1 Smits, Veronique A.J.
A1 Hernández-Carralero, Esperanza
A1 Paz-Cabrera, María Cristina
A1 Cabrera, Elisa
A1 Hernández-Reyes, Yeray
A1 Gillespie, David A.
A1 Salido, Eduardo C.
A1 Hernández-Porto, Miriam
A1 Freire, Raimundo
A2 BioquímicaMicrobiología, Biología Celular y Genética
A2 Unidad de InvestigaciónHospital Universitario de Canarias.
K1 Nucleocapsid protein
K1 Membrane protein
K1 Spike protein
K1 SARS-CoV-2
K1 COVID-19
K1 lmmunotest
AB made to identify infected patients and to detect patients with a positive immune response against thevirus. Currently, attemprs ro generare a vaccine against the coronavirus are ongoing. To understand SARSCoV-2 immunoreactivity, we compared the lgG antibody response against SARS-CoV-2 in infected versuscontrol patients by dot blot using recombinant viral particle proteins: N (Nucleocapsid ). M (Membrane)and S (Spike). In addition, we used difTerent prorein fragments of the N and S protein to map immuneepitopes. Most of the COVID-19 patients presented a speci fic immune response against the full lengthand fragments of the N protein and. to lesser exten t. against a fragment containing amino acids 300- 685of the S protein. In contrast, immunoreacrivity against other S protein fragments or the M prorein waslow. This response is speci fic for COVID-19 patien ts as very few of the control patients displayedimmunoreactiviry, like ly reflecting an immune response against other coronaviruses. Altogether. ourresults may help develop method(s) for measuring COVID-19 antibody response, selectivity of methodsdetecting such SARS-CoV-2 antibodies and vaccine development.
YR 2021
FD 2021
LK http://riull.ull.es/xmlui/handle/915/38848
UL http://riull.ull.es/xmlui/handle/915/38848
LA en
NO DOI: 10.1016/j.bbrc.2021.01.073
DS Repositorio institucional de la Universidad de La Laguna
RD 19-oct-2024