RT info:eu-repo/semantics/article
T1 Acanthamoeba interactions with human brain microvascular endothelial cells
A1 Ortega Rivas, Antonio
A1 Alsam, Selwa
A1 Kim, Kwang Sik
A1 Stins, Monique
A1 Sissons, James
A1 Khan, Naveed Ahmed
A2 Didácticas Específicas
A2 Instituto de Enfermedades Tropicales y Salud Pública de Canarias
K1 Acanthamoeba
K1 Granulomatous amoebic encephalitis
K1 Pathogenic protozoa
K1 Mannose-binding protein
K1 Adhesion
K1 Cytotoxicity
AB Acanthamoeba are opportunistic protozoan parasites that can cause fatal granulomatous amoebic encephalitis, however, the pathogenic mechanisms associated with this disease remain unclear. One of the primary factors in Acanthamoeba encephalitis is the haematogenous spread, followed by invasion of the blood–brain barrier resulting in the transmigration of Acanthamoeba into the central nervous system. In this study, we have used human brain microvascular endothelial cells, which constitute the blood–brain barrier and studied their interactions with Acanthamoeba. Using in vitro cultures, we showed that Acanthamoeba isolates belonging to genotypes T3, T4 and T11, exhibited increased cytotoxicity on human brain microvascular endothelial cells as well as exhibited higher binding and were considered potential pathogens. In contrast, Acanthamoeba isolates belonging to genotypes T2 and T7 exhibited minimal cytotoxicity and significantly less binding to human brain microvascular endothelial cells ðP , 0:01Þ: Furthermore, exogenous a-mannose inhibited binding but increased cytotoxicity of human brain microvascular endothelial cells. This is the first demonstration of Acanthamoeba interactions with primary human brain microvascular endothelial cells.
YR 2003
FD 2003
LK http://riull.ull.es/xmlui/handle/915/38850
UL http://riull.ull.es/xmlui/handle/915/38850
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 23-nov-2024