RT info:eu-repo/semantics/article
T1 Development of a rapid polymerase chain reaction-ELISA assay using polystyrene beads for the detection of Toxoplasma gondii DNA
A1 Ortega Rivas, Antonio
A1 Martínez Carretero, Enrique
A1 Carmelo, Emma
A1 Alonso, R.
A1 Piñero Barroso, José Enrique
A1 del Castillo Remiro, Antonio
A1 Valladares Hernández, Basilio
A2 Didácticas Específicas
K1 colourimetric detection
K1 nested-PCR-ELISA
K1 polystyrene beads
K1 T. gondii
AB Aims: To develop a rapid colourimetric assay for the detection of Toxoplasma gondii DNA using polystyrene beads as solid support. Methods and Results: A nested-polymerase chain reaction (PCR)-ELISA assay for the detection of T. gondii DNA was standardized by optimizing the hybridization time and probe concentration. Its detection threshold was then determined and compared with Southern blotting hybridization. These were found to be equivalent, but the PCR-ELISA-beads test is easier to perform and the turnaround time is much shorter than with Southern blot. Conclusions: The PCR-ELISA-beads assay is a valuable tool for the detection of T. gondii DNA. Significance and Impact of the Study: Our results demonstrate that this PCR-ELISA assay, using polystyrene beads, can be used as a routine diagnostic test for the detection of T. gondii in clinical laboratories.
YR 2003
FD 2003
LK http://riull.ull.es/xmlui/handle/915/38851
UL http://riull.ull.es/xmlui/handle/915/38851
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 22-dic-2024