RT info:eu-repo/semantics/article
T1 A Specific Primer Pair for the Diagnosis and Identification of Acanthamoeba astronyxis by Random Amplified Polymorphic DNA-Polymerase Chain Reaction
A1 Ortega Rivas, Antonio
A1 Lorenzo Morales, Jacob
A1 Martínez Carretero, Enrique
A1 Villa, M.
A1 Clavel, A.
A1 Valladares Hernández, Basilio
A1 del Castillo Remiro, Antonio
A2 Didácticas Específicas
A2 Instituto de Enfermedades Tropicales y Salud Pública de Canarias
AB Random amplified polymorphic DNA (RAPD) is a useful tool for species identification. The obtained band patterns can be used for specific primer pair design that is useful for species identification. In this study, a distinctive 485-bp band in Acanthamoeba astronyxis band patterns was found, using the OPC20 primer (ACTTCGCCAC). The band specificity was con firmed by hybridization, using it as a probe, against all OPC20 amplifications from different Acanthamoeba species. Once the fragment was sequenced, we used it to design a specific primer pair that was useful for the identification of different isolates as A. astronyxis species
YR 2005
FD 2005
LK http://riull.ull.es/xmlui/handle/915/38853
UL http://riull.ull.es/xmlui/handle/915/38853
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DS Repositorio institucional de la Universidad de La Laguna
RD 27-nov-2024