RT info:eu-repo/semantics/article T1 Molecular identification of Hartmannella vermiformis and Vannella persistens from man-made recreational water environments, Tehran, Iran A1 Ortega Rivas, Antonio A1 Nazar, Mahdieh A1 Haghighi, Ali A1 Taghipour, Niloofar A1 Tahvildar-Biderouni, Farid A1 Mojarad, Ehsan Nazemalhosseini A1 Eftekhar, Mohammad A2 Didácticas Específicas A2 Instituto de Enfermedades Tropicales y Salud Pública de Canarias AB A survey was conducted on man-made recreational water located in different regions of Tehran, Iran to detect the free-living amoebae present in ponds and fountains of parks and squares. Fifty water samples from 22 municipal districts of Tehran were screened for free-living amoebae and identified by morphological characters and polymerase chain reaction amplification. Amoebae detected were identified as Hartmannella vermiformis (12 %) and Vannella persistens (4 %), which are the first reports of these two amoebas in recreational water environments of Iran. Since, H. vermiformis, which is highly similar to strains serving as hosts for Legionella pneumophila, is a common component of the microbial community in fresh surface water. Although Vannella spp. is not proved to be pathogenic itself, they are capable of harboring pathogenic intracellular organisms. Due to some reports related to pathogenicity of these amoebas, the particular hazard related to these microorganisms should be taken into account in the encounter with drinking and washing in these waters. We recommend control strategies based on physical removal rather than on disinfection to be adopted where necessary. YR 2012 FD 2012 LK http://riull.ull.es/xmlui/handle/915/38856 UL http://riull.ull.es/xmlui/handle/915/38856 LA en DS Repositorio institucional de la Universidad de La Laguna RD 27-dic-2024