RT info:eu-repo/semantics/article
T1 Design and evaluation of a specific primer pair for the diagnosis and identification of Acanthamoeba polyphaga
A1 Ortega Rivas, Antonio
A1 Lorenzo Morales, Jacob
A1 Martínez Carretero, Enrique
A1 Villa Lloberas, Mercedes
A1 Valladares Hernández, Basilio
A1 del Castillo Remiro, Antonio
A2 Didácticas Específicas
A2 Instituto de Enfermedades Tropicales y Salud Pública de Canarias
AB Random amplified polymorphism DNA (RAPD) is a useful tool for species identification. The obtained band patterns can be used for specific primer pair design that may be useful for species diagnosis. In this study, a distinctive a 962-bp band in A. polyphaga band patterns was found, by using the OPC20 primer (ACTTCGCCAC). The DNA fragment was used to design a specific primer pair that was useful for the identification of different isolates as A. polyphaga species. A case of A. polyphaga in disseminated acanthamoebiasis affecting mesenteric nodes is also reported.
YR 2004
FD 2004
LK http://riull.ull.es/xmlui/handle/915/38857
UL http://riull.ull.es/xmlui/handle/915/38857
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DS Repositorio institucional de la Universidad de La Laguna
RD 16-oct-2024