RT info:eu-repo/semantics/article
T1 Evaluation of prokaryotic and eukaryotic cells as food source for Balamuthia mandrillaris
A1 Ortega Rivas, Antonio
A1 Matin, Abdul
A1 Jeong, Seok Ryoul
A1 Faull, Jane
A1 Khan, Naveed Ahmed
A2 Didácticas Específicas
A2 Instituto de Enfermedades Tropicales y Salud Pública de Canarias
K1 Balamuthia mandrillaris
K1 Feeding
K1 Brain endothelial cells
K1 Bacteria
K1 Encephalitis
AB Balamuthia mandrillaris is a recently identiWed free-living protozoan pathogen that can cause fatal granulomatous encephalitis in humans. Recent studies have shown that B. mandrillaris consumes eukaryotic cells such as mammalian cell cultures as food source. Here, we studied B. mandrillaris interactions with various eukaryotic cells including, monkey kidney Wbroblast-like cells (COS-7), human brain microvascular endothelial cells (HBMEC) and Acanthamoeba (an opportunistic protozoan pathogen) as well as prokaryotes, Escherichia coli. B. mandrillaris exhibited optimal growth on HBMEC compared with Cos-7 cells. In contrast, B. mandrillaris did not grow on bacteria but remained in the trophozoite stage. When incubated with Acanthamoeba trophozoites, B. mandrillaris produced partial Acanthamoeba damage and the remaining Acanthamoeba trophozoites underwent encystment. However, B. mandrillaris were unable to consume Acanthamoeba cysts. Next, we observed that B. mandrillaris-mediated Acanthamoeba encystment is a contactdependent process that requires viable B. mandrillaris. In support, conditioned medium of B. mandrillaris did not stimulate Acanthamoeba encystment nor did lysates of B. mandrillaris. Overall, these studies suggest that B. mandrillaris target Acanthamoeba in the trophozoite stage; however, Acanthamoeba possess the ability to defend themselves by forming cysts, which are resistant to B. mandrillaris. Further studies will examine the mechanisms associated with food selectivity in B. mandrillaris.
YR 2006
FD 2006
LK http://riull.ull.es/xmlui/handle/915/38861
UL http://riull.ull.es/xmlui/handle/915/38861
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 24-nov-2024