RT info:eu-repo/semantics/article
T1 Random amplified polymorphic DNA profiles as a tool for the identification of Acanthamoeba divionensis
A1 Ortega Rivas, Antonio
A1 Lorenzo Morales, Jacob
A1 Alonso, Violeta
A1 Abreu Acosta, Néstor
A1 Foronda, Pilar
A1 del Castillo Remiro, Antonio
A1 Valladares Hernández, Basilio
A2 Didácticas Específicas
A2 Instituto de Enfermedades Tropicales y Salud Pública de Canarias
AB In the present study, we demonstrated the Random Amplified Polymorphism DNA (RAPD) diagnostic validity. In our study, we have analyzed RAPD profiles searching for characteristic and useful bands for Acanthamoeba diagnosis at the species level. We found a distinctive 370-bp band in A. divionensis RAPD patterns, using the OPC14 primer (TGCGTGCTTG) (Operon Technologies, Inc., Alameda, CA). The band specificity was confirmed by hybridization, using it as a probe, against all OPC14 amplifications from 10 different Acanthamoeba species. Once we sequenced this band, we used it to design a specific primer pair which showed positive amplification only in A. divionensis isolates.
YR 2003
FD 2003
LK http://riull.ull.es/xmlui/handle/915/38866
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DS Repositorio institucional de la Universidad de La Laguna
RD 27-dic-2024