RT info:eu-repo/semantics/article
T1 Novel regulation from novel interactions: Identification of an RNA sponge that controls the levels, processing and efficacy of the RoxS riboregulator of central metabolism in Bacillus subtilis
A1 Hernández Fernaud, Juan Ramón
A1 Durand, Sylvain
A1 Callan-Sida, Adam
A1 McKeown, Josie
A1 Li, Stephen
A1 Kostova, Gergana
A1 Alam, Mohammad Tauqeer
A1 Millard, Andrew
A1 Allouche, Delphine
A1 Constantinidou, Chrystala
A1 Condon, Ciaran
A1 Denham, Emma L.
A2 BioquímicaMicrobiología, Biología Celular y Genética
A2 School of Life Sciences. University of Warwick.
AB sRNAs are a taxonomically-restricted buttranscriptomically-abundant class of posttranscriptional regulators. While of major importance for adaption to the environment, wecurrently lack global-scale methodology enablingtarget identification, especially in species withoutknown RNA hub proteins (e.g. Hfq). Using psoralenRNA cross-linking and Illumina-sequencing weidentify RNA–RNA interacting pairs in vivo in Bacillus subtilis, resolving previously well-describedinteractants. Although sRNA–sRNA pairings arerare (compared with sRNA–mRNA), we identify arobust example involving the conserved sRNA RoxSand an unstudied sRNA RosA (Regulator of sRNAA). We show RosA to be the first confirmed RNAsponge described in a Gram-positive bacterium.RosA interacts with at least two sRNAs, RoxS andFsrA. The RosA/RoxS interaction not only affectsthe levels of RoxS but also its processing and regulatory activity. We also found that the transcriptionof RosA is repressed by CcpA, the key regulatorof carbon-metabolism in B. subtilis. Since RoxSis already known to be transcriptionally controlledby malate via the transcriptional repressor Rex, itspost-transcriptional regulation by CcpA via RosA places RoxS in a key position to control centralmetabolism in response to varying carbon sources.
YR 2021
FD 2021
LK http://riull.ull.es/xmlui/handle/915/38872
UL http://riull.ull.es/xmlui/handle/915/38872
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DS Repositorio institucional de la Universidad de La Laguna
RD 31-oct-2024