RT info:eu-repo/semantics/article
T1 Use of In Vitro Assays To Determine Effects of Human Serum on Biological Characteristics of Acanthamoeba castellanii.
A1 Ortega Rivas, Antonio
A1 Sissons, James
A1 Alsam, Selwa
A1 Stins, Monique
A1 Lorenzo Morales, Jacob
A1 Faull, Jane
A1 Khan, Naveed Ahmed
A2 Didácticas Específicas
A2 Instituto de Enfermedades Tropicales y Salud Pública de Canarias
AB Normal human serum inhibits Acanthamoeba (encephalitis isolate) binding to and cytotoxicity of human brain microvascular endothelial cells, which constitute the blood-brain barrier. Zymographic assays revealed that serum inhibits extracellular protease activities of acanthamoebae. But it is most likely that inhibition of specific properties of acanthamoebae is a consequence of the initial amoebicidal-amoebistatic effects induced by serum. For example, serum exhibited amoebicidal effects; i.e., up to 50% of the exposed trophozoites were killed. The residual subpopulation, although viable, remained static over longer incubations. Interestingly, serum enhanced the phagocytic ability of acanthamoebae, as measured by bacterial uptake. Overall, our results demonstrate that human serum has inhibitory effects on Acanthamoeba growth and viability, protease secretions, and binding to and subsequent cytotoxicity for brain microvascular endothelial cells. Conversely, Acanthamoeba phagocytosis was stimulated by serum.
YR 2006
FD 2006
LK http://riull.ull.es/xmlui/handle/915/38874
UL http://riull.ull.es/xmlui/handle/915/38874
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 24-nov-2024