RT info:eu-repo/semantics/article
T1 The Na, K-ATPase -Subunit Isoforms Expression in Glioblastoma Multiforme: Moonlighting Roles
A1 Morales González, Manuel José
A1 Rotoli, Deborah
A1 Cejas, Mariana-Mayela
A1 Maeso, María-del-Carmen
A1 Pérez-Rodríguez, Natalia-Dolores
A1 Ávila, Julio
A1 Mobasheri, Ali
A1 Martín-Vasallo, Pablo
A2 Medicina InternaDermatología y Psiquiatría
K1 Glioblastoma multiforme
K1 NA, K-ATPase
K1 sodium pump
K1 Na, K-ATPase β subunit
isoforms
K1 moonlighting proteins
K1 β2/AMOG
K1 Glioblastoma multiforme microenvironment
K1 astrocyte-neuron adhesion
K1 Two-Hybrid system
AB Glioblastoma multiforme (GBM) is the most common form of malignant glioma.Recent studies point out that gliomas exploit ion channels and transporters, including Na,K-ATPase, to sustain their singular growth and invasion as they invade the brain parenchyma.Moreover, the different isoforms of the β-subunit of Na, K-ATPase have been implicated in regulatingcellular dynamics, particularly during cancer progression. The aim of this study was to determinethe Na, K-ATPase β subunit isoform subcellular expression patterns in all cell types responsible formicroenvironment heterogeneity of GBM using immunohistochemical analysis. All three isoforms,β1, β2/AMOG (Adhesion Molecule On Glia) and β3, were found to be expressed in GBM samples.Generally, β1 isoform was not expressed by astrocytes, in both primary and secondary GBM,although other cell types (endothelial cells, pericytes, telocytes, macrophages) did express thisisoform. β2/AMOG and β3 positive expression was observed in the cytoplasm, membrane andnuclear envelope of astrocytes and GFAP (Glial Fibrillary Acidic Protein) negative cells. Interestingly,differences in isoforms expression have been observed between primary and secondary GBM:in secondary GBM, β2 isoform expression in astrocytes was lower than that observed in primaryGBM, while the expression of the β3 subunit was more intense. These changes in β subunit isoformsexpression in GBM could be related to a different ionic handling, to a different relationship betweenastrocyte and neuron (β2/AMOG) and to changes in the moonlighting roles of Na, K-ATPase βsubunits as adaptor proteins and transcription factors
YR 2017
FD 2017
LK http://riull.ull.es/xmlui/handle/915/40036
UL http://riull.ull.es/xmlui/handle/915/40036
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DS Repositorio institucional de la Universidad de La Laguna
RD 28-nov-2024