RT info:eu-repo/semantics/article
T1 Pitavastatin loaded nanoparticles: A suitable ophthalmic treatment for Acanthamoeba Keratitis inducing cell death and autophagy in Acanthamoeba polyphaga
A1 Oliva Martín, Alexis Manuel
A1 Sifaoui, Ines
A1 Díaz Rodríguez, Patricia
A1 Rodríguez Expósito, Rub´en L.
A1 Reyes-Batlle, María
A1 López Arencibia, Atteneri
A1 Salazar Villatoro, Lizbeth
A1 Castelan Ramírez, Ismael
A1 Omaña Molina, Maritza
A1 Piñero Barroso, José Enrique
A1 Lorenzo Morales, Jacob
A2 Ingeniería Química y Tecnología Farmacéutica
A2 Instituto de Enfermedades Tropicales y Salud Pública de Canarias
K1 Acanthamoeba spp
K1 Polymeric Nanoparticles
K1 Pitavastatin
K1 Tubulin
K1 Programmed cell death
AB Statins are effective sterol lowering agents with high amoebicidal activity. Nevertheless, due to their pooraqueous solubility, they remain underused especially in eye drop formulation. The aim of the present study is todevelop Pitavastatin loaded nanoparticles suitable for ophthalmic administration and designed for the managementof Acanthamoeba Keratitis. These nanocarriers are aimed to solve both the ophthalmic route-associatedproblems and the limited aqueous drug solubility issues of Pitavastatin. Nanoparticles were obtained by ananoprecipitation-solvent displacement method and their amoebicidal activity was evaluated against four strainsof Acanthamoeba: A. castellanii Neff, A. polyphaga, A. griffini and A. quina. In Acanthamoeba polyphaga, the effect ofthe present nanoparticles was investigated with respect to the microtubule distribution and several programmedcell death features. Nanoparticles were able to eliminate all the tested strains and Acanthamoeba polyphaga wasdetermined to be the most resistance strain. Nanoparticles induced chromatin condensation, autophagic vacuolesand mitochondria dysfunction
YR 2022
FD 2022
LK http://riull.ull.es/xmlui/handle/915/40115
UL http://riull.ull.es/xmlui/handle/915/40115
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 24-nov-2024