RT info:eu-repo/semantics/article
T1 SEGS-1 a cassava genomic sequence increases the severity of African cassava mosaic virus infection in Arabidopsis thaliana.
A1 León Guerra, Leandro Jesús de
A1 Rajabu, Cyprian A.
A1 Dallas, Mary M.
A1 Chiunga, Evangelista
A1 Ateka, Elijah M.
A1 Tairo, Fred
A1 Ndunguru, Joseph
A1 Ascencio Ibáñez, José T.
A1 Hanley-Bowdoin, Linda
A2 BioquímicaMicrobiología, Biología Celular y Genética
K1 SEGS-1
K1 begomovirus
K1 ACMV
K1 Arabidopsis thaliana
K1 cassava
AB Cassava is a major crop in Sub-Saharan Africa, where it is grown primarily by smallholder farmers. Cassava production is constrained by Cassava mosaic disease (CMD), which is caused by a complex of cassava mosaic begomoviruses (CMBs). A previous study showed that SEGS-1 (sequences enhancing geminivirus symptoms), which occurs in the cassava genome and as episomes during viral infection, enhances CMD symptoms and breaks resistance in cassava. We report here that SEGS-1 also increases viral disease severity in Arabidopsis thaliana plants that are co-inoculated with African cassava mosaic virus (ACMV) and SEGS-1 sequences. Viral disease was also enhanced in Arabidopsis plants carrying a SEGS-1 transgene when inoculated with ACMV alone. Unlike cassava, no SEGS-1 episomal DNA was detected in the transgenic Arabidopsis plants during ACMV infection. Studies using Nicotiana tabacum suspension cells showed that co-transfection of SEGS-1 sequences with an ACMV replicon increases viral DNA accumulation in the absence of viral movement. Together, these results demonstrated that SEGS-1 can function in a heterologous host to increase disease severity. Moreover, SEGS-1 is active in a host genomic context, indicating that SEGS-1 episomes are not required for disease enhancement.
YR 2023
FD 2023
LK http://riull.ull.es/xmlui/handle/915/40736
UL http://riull.ull.es/xmlui/handle/915/40736
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 27-abr-2025