RT info:eu-repo/semantics/article
T1 Atp: The crucial component of secretory vesicles
A1 Estévez Herrera, Judith
A1 Domínguez, Natalia
A1 Pardo, Marta R.
A1 González Santana, Ayoze
A1 Westhead, Edward W.
A1 Borges Jurado, Ricardo
A1 Machado Ponce, José David
A2 Medicina Física y Farmacología
A2 Grupo de investigación: Exocitosis
K1 exocytosis
K1 purines
K1 quantum size
K1 secretory vesicles
K1 VNUT
AB The colligative properties of ATP and catecholamines demonstrated in vitro are thought to be responsible for the extraordinary accumulation of solutes inside chromaffin cell secretory vesicles, although this has yet to be demonstrated in living cells. Because functional cells cannot be deprived of ATP, we have knocked down theexpression ofthe vesicular nucleotide carrier, the VNUT,to show that a reduction in vesicular ATP is accompanied by a drastic fall in the quantal release of catecholamines. This phenomenon is particularly evident in newly synthesized vesicles, which we show are the first to be released. Surprisingly, we find that inhibiting VNUT expression also reduces the frequency of exocytosis, whereas the overexpression of VNUT drastically increases the quantal size of exocytotic events. To our knowledge, our data provide the first demonstration that ATP, in addition to serving as an energy source and purinergic transmitter, is an essential element in the concentration of catecholamines in secretory vesicles. In this way, cells can use ATP to accumulate neurotransmitters and other secreted substances at high concentrations, supporting quantal transmission.
YR 2016
FD 2016
LK http://riull.ull.es/xmlui/handle/915/41334
UL http://riull.ull.es/xmlui/handle/915/41334
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 26-abr-2025