RT info:eu-repo/semantics/article
T1 Fluoroquinolone antibiotic determination in bovine, ovine and caprine milk using solid-phase extraction and high-performance liquid chromatography-fluorescence detection with ionic liquids as mobile phase additives
A1 Herrera Herrera, Antonio Vicente
A1 Hernández Borges, Javier
A1 Rodríguez Delgado, Miguel Ángel
A2 Química
A2 Análisis Químico Aplicado a la IndustriaMedio ambiente y Productos Agroalimentarios (AQAIMPA)
K1 High-performance liquid chromatography
K1 fluorescence detection
K1 fluoroquinolones
K1 antibiotics
K1 ionic liquids,
K1 solid-phase extraction
K1 milk
AB This paper describes the use of 1-ethyl-3-methylimidazolium tetrafluoroborate (EMIm-BF4) as mobile phase additive for the high-performance liquid chromatography with fluorescence detection analysis of a group of seven basic fluoroquinolone antibiotics (i.e. fleroxacin, ciprofloxacin, lomefloxacin, danofloxacin, enrofloxacin,sarafloxacin and difloxacin) in different milk samples. EMIm-BF4 was found superior to 1-butyl-3-methylimidazolium tetrafluoroborate for the separation of the analytes fromchromatographic interferences of the sample matrix. The optimized method was applied to the analysis of ovine, caprine and bovine milk, in the last case in either skimmed, semi-skimmed and full-cream milk after suitable acidic deproteination followed by a solid-phase extraction procedure. Recovery values between 73% and 113% were obtained for the three types of bovine milk samples, as well as for ovine and caprine milk (RSDs below 16% in all cases), which clearly demonstrates the applicability of the method to the three types of milk no matter the fat content of the samples. Limits of  detection were in the range 0.5-8.1 µg/L (approx 0.5-25.9 µg/kg), well below the maximum residue limits established for these compounds by the current European legislation. A screening study of 24 different milk samples was also developed. In none of the samples, residues of the selected antibiotics were found.
YR 2009
FD 2009
LK http://riull.ull.es/xmlui/handle/915/41402
UL http://riull.ull.es/xmlui/handle/915/41402
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 05-may-2025