RT info:eu-repo/semantics/article
T1 Antifeedant, antifungal and nematicidal compounds from the endophyte Stemphylium solani isolated from wormwood
A1 Giménez Mariño, Cristina
A1 Díaz, Carmen E.
A1 Andrés, María Fe
A1 Lacret, Rodney
A1 Cabrera Pérez, Raimundo Manuel
A1 Kaushik, Nutan
A1 González Coloma, Azucena
A2 BotánicaEcología y Fisiología Vegetal
K1 Antifeedant
K1 antifungal
K1 nematicidal compounds
K1 endophyte
K1 Stemphylium solani
AB The continuous search for natural product-based biopesticides from fungi isolated from untapped sources is an efective tool. In this study, we studied a pre-selected fungal endophyte, isolate Aa22, from the medicinal plant Artemisia absinthium, along with the antifungal, insect antifeedant andnematicidal compounds present in the extract. The endophyte Aa22 was identifed as Stemphylium solani by molecular analysis. The antifungal activity was tested by broth microdilution against Fusarium solani, F. oxysporum, F. moniliforme and Botrytis cinerea, the insect antifeedant by choice bioassays against Spodoptera littoralis, Myzus persicae and Rhopalosiphum padi and the in vitromortality against the root-knot nematode Meloiydogyne javanica. The structures of bioactive compounds were determined on the basis of 1D and 2D NMR spectroscopy and mass spectrometry.The ethyl acetate extract obtained from the solid rice fermentation showed mycelial growth inhibition of fungal pathogens (EC50 0.08–0.31 mg/mL), was antifeedant to M. persicae (99%) and nematicidal (68% mortality). A bioguided fractionation led to the isolation of the new compound stempholone A (1), and the known stempholone B (2) and stemphol (3). These compounds exhibited antifeedant(EC50 0.50 mg/mL), antifungal (EC50 0.02–0.43 mg/L) and nematicidal (MLD 0.5 mg/mL) activities. The extract activities can be explained by 3 (antifungal), 1–3 (antifeedant) and 1 (nematicidal). Phytotoxicity tests on Lolium perenne and Lactuca sativa showed that the extract and 1 increased L. sativa root growth (121–130%) and 1 reduced L. perenne growth (48–49%). These results highlight the potential of the endophytic fungi Aa22 as biotechnological source of natural product-based biopesticides.
YR 2024
FD 2024
LK http://riull.ull.es/xmlui/handle/915/41455
UL http://riull.ull.es/xmlui/handle/915/41455
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 26-abr-2025