RT info:eu-repo/semantics/article
T1 Comparative assessment of microsatellite and retrotransposon-based markers for genetic characterization of commercial banana cultivars (Musa spp.)
A1 González Carracedo, Mario Andrés
A1 Tejera Pérez, Hugo
A1 Hernández Ferrer, Mariano
A1 Jiménez Arias, David
A1 Pérez Pérez, José Antonio
A2 BioquímicaMicrobiología, Biología Celular y Genética
K1 cultivars
K1 iPBS
K1 LTR-retrotransposon
K1 microsatellite
K1 Musa
K1 SSR
AB Banana cultivars of agronomic interest have been genetically characterized using two different molecular markers. On the one hand, a panel of 14 trinucleotide single sequence repeats (SSRs or microsatellites) was optimized for homogeneous PCR conditions. It was tested with 50 individuals from seven cultivars, yielding 76 alleles and 5.4 ± 1.8 alleles per locus, while the presence of cultivar-exclusive alleles allowed the discrimination of all cultivars. On the other hand, a retrotransposon-based marker system named inter-primer binding site (iPBS) was implemented for the first time in the Musa genus. A total of 120 bands were detected in eight different Musa cultivars, from which 65.8% were polymorphic and 23.3% were cultivar exclusive. Both techniques allowed a cut-off identification of all cultivars studied, but overall, iPBS analysis was a more straightforward and economical choice. Despite the fact that we were unable to distinguish local banana varieties belonging to the same cultivar, new cultivar-specific molecular markers have been developed for the Musa genus, which could be used to guide new breeding programmes and maintain high quality of Plátano de Canarias.
YR 2021
FD 2021
LK http://riull.ull.es/xmlui/handle/915/41771
UL http://riull.ull.es/xmlui/handle/915/41771
LA en
NO https://portalciencia.ull.es/documentos/6132c4bf33c0636613bd9591
DS Repositorio institucional de la Universidad de La Laguna
RD 17-nov-2025