RT info:eu-repo/semantics/masterThesis
T1 rDNA structure under stress and chromosome remodelling
A1 Matos Perdomo, Emiliano José
A2 Máster Universitario en Biomedicina
K1 Cdc14
K1 Condensación cromosómica
K1 Estrés térmico
K1 rDNA
K1 TORC1
AB Chromosome structure in the yeast Saccharomyces cerevisiae is only visible at themicroscopic level in the chromosome XII. The ribosomal DNA (rDNA) located in thelong arm of this chromosome has been used to observed different phenomena ofcompaction, segregation and chromosomal structure at different phases of the cell cycle.The metaphase structure (“loop”) depends, among others, on the condensin complex;and its segregation during anaphase depends also in that complex and on the cell cyclephosphatase Cdc14. This study aims to elucidate chromosome remodelling under heatstress and other stresses and the influence of Cdc14 and the TOR pathway in this process.For this, I used different tagged proteins that bind to the rDNA locus for their monitoringand visualization with fluorescent microscopy under heat-stress conditions, besides, Iapplied several molecular and immunological techniques. I employed an auxin baseddegron system to degraded proteins by the proteasome. I found that the rDNAchromosome structure is condensed under heat-stress conditions and TORC1 inhibition,also I verified that the auxin mediated degradation system is a useful tool for thispurpose. Finally, a role for TORC1 signalling pathway on the stress mediatedcondensation process, was established.
YR 2018
FD 2018
LK http://riull.ull.es/xmlui/handle/915/7272
UL http://riull.ull.es/xmlui/handle/915/7272
LA es
DS Repositorio institucional de la Universidad de La Laguna
RD 20-abr-2024