Deciphering tacrolimus-induced toxicity in pancreatic ß-cells.

Acebes Vindel, Ángel José; Triñanes, J.; Rodríguez Rodríguez, A.E.; Brito Casillas, Y.; Wagner, A.; De Vries, A.P.J.; Cuesto, G.; Salido, E.; Torres, A.; Porrini, E.

doi:10.1111/ajt.14323

View/Open

Export Citations

Date

2017

URI

http://riull.ull.es/xmlui/handle/915/35809

Abstract

b Cell transcription factors such as fork head box proteinO1(FoxO1), v-maf musculoaponeurotic fibrosarcoma oncogene homologA (MafA), pancreatic and duodenal homeobox1, and neuronal differentiation 1, are dysfunctional in type 2 diabetes mellitus (T2DM). Posttransplant diabetes mellitus resembles T2DMandreflectsinteractionbetweenpretransplant insulin resistanceand immunosuppressants ,mainly calcineurininhibitors(CNIs).We evaluated the effect of tacrolimus(TAC),cyclosporineA(CsA),and metabolic stressors (glucose plus palmitate) on insulinomab cells invitro and in pancreata of obeseand lean Zucker rats.Cellswereculturedfor5dayswith 100lMpalmitateand22mMglucose;CsA(250ng/ mL)orTAC(15ng/mL)were added in the last48h.

Licencia Creative Commons (Reconocimiento-No comercial-Sin obras derivadas 4.0 Internacional)

Except where otherwise noted, this item's license is described as Licencia Creative Commons (Reconocimiento-No comercial-Sin obras derivadas 4.0 Internacional)