RT info:eu-repo/semantics/article
T1 Role of CXCL13 and CCL20 in the recruitment of B cells to inflammatory foci in chronic arthritis
A1 Castro Hernández, Javier
A1 Armas González, Estefanía
A1 Domínguez Luis,  María Jesús
A1 Díaz Martín, Ana
A1 Arce Franco, Mayte
A1 Danelon, Gabriela
A1 Hernández Hernández, Vanesa
A1 Bustabad Reyes, Sagrario
A1 Cantabrana, Alberto
A1 Uguccioni, Mariagrazia
A1 Díaz González, Federico
A2 Medicina Física y Farmacología
K1 Rheumatoid arthritis
K1 Psoriatic arthritis
K1 B cells
K1 Chemokines and chemokine receptors
AB Background: B cells exert their pathogenic action in rheumatoid arthritis (RA) locally in the synovium. This studywas undertaken to elucidate the chemokines responsible for the recruitment of B cells in the inflamed synovium,taking into account that the rich chemokine milieu present in the synovial tissue can fine-tune modulate discretechemokine receptors.Methods: Expression levels of chemokine receptors from the CC and CXC family, as well as CD27, were assessed byflow cytometry in CD20+ mononuclear cells isolated from the peripheral blood (PB) and synovial fluid (SF) of RAand psoriatic arthritis patients. Transwell experiments were used to study migration of B cells in response to achemokine or in the presence of multiple chemokines.Results: B cells from the SF of arthritis patients showed a significant increase in the surface expression of CCR1,CCR2, CCR4, CCR5 and CXCR4 with respect to PB. Conversely, SF B cells expressed consistently lower amounts ofCXCR5, CXCR7 and CCR6, independent of CD27 expression. Analysis of permeabilized B cells suggestedinternalization of CXCR5 and CCR6 in SF B cells. In Transwell experiments, CCL20 and CXCL13, ligands of CCR6 andCXCR5, respectively, caused a significantly higher migration of B cells from PB than of those from SF of RA patients.Together, these two chemokines synergistically increased B-cell migration from PB, but not from SF.Conclusions: These results suggest that CXCL13 and CCL20 might play major roles in RA pathogenesis by actingsingly on their selective receptors and synergistically in the accumulation of B cells within the inflamed synovium.
YR 2018
FD 2018
LK http://riull.ull.es/xmlui/handle/915/34778
UL http://riull.ull.es/xmlui/handle/915/34778
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 22-may-2024