RT info:eu-repo/semantics/article
T1 Fast SARS-CoV-2 detection by RT-qPCR in preheated nasopharyngeal swab samples.
A1 Valenzuela Fernández, Agustín
A1 Alcoba Florez, Julia
A1 González Montelongo, Rafaela
A1 Íñigo Campos, Antonio
A1 García-Martínez de Artola, Diego
A1 Gil Campesino, Helena
A1 The Microbiology Technical Support Team
A1 Ciuffreda, Laura
A1 Flores, Carlos
A2 Medicina Física y Farmacología
A2 Grupo "inmunología Celular y Viral".
K1 COVID-19
K1 SARS-CoV-2
K1 diagnosis
K1 sample treatment
K1 RNA extraction
K1 fast protocols
AB Objectives: The gold-standard COVID-19 diagnosis relies on detecting SARS-CoV-2 using RNA purificationand one-step retrotranscription and quantitative PCR (RT-qPCR). Based on the urgent need for highthroughput screening, we tested the performance of three alternative, simple and affordable protocols torapidly detect SARS-CoV-2, bypassing the long and tedious RNA extraction step and reducing the time toviral detection.Methods: We evaluated three methods based on direct nasopharyngeal swab viral transmission medium(VTM) heating before the RT-qPCR: a) direct without additives; b) in a formamide-EDTA (FAE) buffer, c) ina RNAsnapTM buffer.Results: Although with a delay in cycle threshold compared to the gold-standard, we found consistentresults in nasopharyngeal swab samples that were subject to a direct 70!C incubation for 10 min.Conclusions: Our findings provide valuable options to overcome any supply chain issue and help toincrease the throughput of diagnostic tests, thereby complementing standard diagnosis
YR 2020
FD 2020
LK http://riull.ull.es/xmlui/handle/915/35354
UL http://riull.ull.es/xmlui/handle/915/35354
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 06-jun-2024